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CEA Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - CEA Antibody (Center) AP6513c
    All lanes : Anti-CEA Antibody (Center) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HT-29 whole cell lysate Lane 3: PC-3 whole cell lysate Lane 4: A549 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 77 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - CEA Antibody (Center) AP6513c
    CEA Antibody (Center) (Cat. #AP6513c) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CEA Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
  • 4 - CEA Antibody (Center) AP6513c
    CEA Antibody (Center) (Cat. #AP6513c) flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, FC, E
Primary Accession P06731
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 76795 Da
Additional info
Gene ID 1048
Other Names Carcinoembryonic antigen-related cell adhesion molecule 5, Carcinoembryonic antigen, CEA, Meconium antigen 100, CD66e, CEACAM5, CEA
Target/Specificity This CEA antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 400-429 amino acids from the Central region of human CEA.
Dilution WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsCEA Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name CEACAM5
Synonyms CEA
Function Cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. Receptor for E.coli Dr adhesins.
Cellular Location Cell membrane; Lipid-anchor, GPI-anchor
Tissue Location Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon
Research Areas

BACKGROUND

CEA is a cell surface glycoprotein that plays a role in cell adhesion and in intracellular signaling. It is a receptor for E.coli Dr adhesins.

REFERENCES

Lagoudianakis,E.E., J. Surg. Res. 155 (2), 183-190 (2009)
Gambichler,T., Am. J. Clin. Pathol. 131 (6), 782-787 (2009)

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