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|Application ||WB, E|
|Other Names||Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, urinary form, Monocyte differentiation antigen CD14, membrane-bound form, CD14|
|Target/Specificity||This CD14 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 54-83 amino acids from the N-terminal region of human CD14.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD14 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the MD-2/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.|
|Cellular Location||Cell membrane; Lipid-anchor, GPI-anchor.|
|Tissue Location||Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages|
Author : Ishida A1, Akita K1, Mori Y1, Tanida S1, Toda M1, Inoue M1, Nakada H2.
J Biol Chem. 2014 Sep 5;289(36):25341-50. doi: 10.1074/jbc.M113.523480. Epub 2014 Jul 24.
Provided below are standard protocols that you may find useful for product applications.
CD14 is a surface protein preferentially expressed on monocytes/macrophages. It binds lipopolysaccharide binding protein and recently has been shown to bind apoptotic cells.
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