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ATF4 Antibody (S245)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ATF4 Antibody (S245) AP6287a
    Anti-ATF4 Antibody (S245) at 1:1000 dilution + K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 39 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ATF4 Antibody (S245) AP6287a
    Western blot analysis of ATF4 (arrow) using rabbit anti-ATF4 Antibody (S245) (Cat. #AP6287a). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the ATF4 gene (Lane 2) (Origene Technologies).
  • 1 - ATF4 Antibody (S245) AP6287a
    Western blot analysis of ATF4 Antibody (S245) Pab (Cat. #AP6287a) pre-incubated without(lane 1) and with(lane 2) blocking peptide in WiDr cell line lysate. ATF4 Antibody (pS245) (arrow) was detected using the purified Pab.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession Q96AQ3
Other Accession P18848
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Other Names Tax-responsive enhancer element B67; ATF4 protein
Target/Specificity This ATF4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 220-252 amino acids from human ATF4.
Dilution WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsATF4 Antibody (S245) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Research Areas

BACKGROUND

ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV-1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB-2). ATF4 belongs to a family of DNA-binding proteins that includes the AP-1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain.

REFERENCES

Gombart,A.F., J. Leukoc. Biol. 81 (6), 1535-1547 (2007)
Jousse,C., J. Biol. Chem. 282 (21), 15851-15861 (2007)
Kakiuchi,C., Neurosci. Lett. 417 (3), 316-321 (2007)
Marchand,A., J. Biol. Chem. 281 (28), 19124-19133 (2006)

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