注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   精选抗体   >   MEF2C Antibody (S387)   

MEF2C Antibody (S387)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
标注"皇冠"的产品,是满足高质量标准的产品。
了解更多...
  • 1 - MEF2C Antibody (S387) AP6285a
    All lanes : Anti-MEF2C-pS387. ctrl at 1:1000-2000 dilution Lane 1: K562 whole cell lysate Lane 2: RPMI 8226 whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 51 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 3 - MEF2C Antibody (S387) AP6285a
    Fluorescent confocal image of Hela cell stained with MEF2C Antibody (S387)(Cat#AP6285a).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEF2C primary antibody (1:25, 1 h at 37℃). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37℃).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).MEF2C immunoreactivity is localized to vesicles and Nucleus significantly.
  • 14 - MEF2C Antibody (S387) AP6285a
    Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C Antibody (S387) (Cat.#AP6285a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, IF, E
Primary Accession Q06413
Other Accession A4UTP7
Reactivity Human, Mouse
Predicted Pig
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 51221 Da
Additional info
Gene ID 4208
Other Names Myocyte-specific enhancer factor 2C, MEF2C
Target/Specificity This MEF2C antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-394 amino acids from human MEF2C.
Dilution WB~~1:1000-2000
IF~~1:10~50
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMEF2C Antibody (S387) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MEF2C
Function Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle- specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. Plays an essential role in hippocampal-dependent learning and memory by suppressing the number of excitatory synapses and thus regulating basal and evoked synaptic transmission. Crucial for normal neuronal development, distribution, and electrical activity in the neocortex. Necessary for proper development of megakaryocytes and platelets and for bone marrow B-lymphopoiesis. Required for B-cell survival and proliferation in response to BCR stimulation, efficient IgG1 antibody responses to T-cell-dependent antigens and for normal induction of germinal center B-cells. May also be involved in neurogenesis and in the development of cortical architecture (By similarity). Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2.
Cellular Location Nucleus.
Tissue Location Expressed in brain and skeletal muscle.
Research Areas

BACKGROUND

MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. This protein controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It may also be involved in neurogenesis and in the development of cortical architecture.

REFERENCES

Konig, S., et al., J. Biol. Chem. 279(27):28187-28196 (2004). Maeda, T., et al., J. Biol. Chem. 277(50):48889-48898 (2002). Maeda, T., et al., Biochem. Biophys. Res. Commun. 294(4):791-797 (2002). Janson, C.G., et al., Brain Res. Mol. Brain Res. 97(1):70-82 (2001). Krainc, D., et al., Genomics 29(3):809-811 (1995).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价