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INA (alpha internexin) Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - INA (alpha internexin) Antibody (Center) AP6284c
    All lanes : Anti-INA Antibody (Center) at 1:2000 dilution Lane 1: Human brain tissue lysate Lane 2: Mouse brain tissue lysate Lane 3: Rat brain tissue lysate Lane 4: SH-SY5Y whole cell lysate Lane 5: Human liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - INA (alpha internexin) Antibody (Center) AP6284c
    All lanes : Anti-INA Antibody (Center) at 1:1000-2000 dilution Lane 1: Human brain tissue lysate Lane 2: U-87 MG whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: Mouse liver tissue lysate Lane 5: Mouse brain tissue lysate Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 55 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - INA (alpha internexin) Antibody (Center) AP6284c
    Formalin-fixed and paraffin-embedded human brain tissue reacted with INA antibody (Center) (Cat.#AP6284c), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q16352
Other Accession P23565, P46660, Q08DH7
Reactivity Human, Mouse, Rat
Predicted Mouse, Rat, Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 9118
Other Names Alpha-internexin, Alpha-Inx, 66 kDa neurofilament protein, NF-66, Neurofilament-66, Neurofilament 5, INA, NEF5
Target/Specificity This INA (alpha internexin) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 290-319 amino acids from the Central region of human INA (alpha internexin).
Dilution WB~~1:1000-2000
IHC-P~~1:10~50
Format Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsINA (alpha internexin) Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name INA
Synonyms NEF5
Function Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.
Tissue Location Found predominantly in adult CNS.
Research Areas
EGFR Amplification and IDH Mutations in Glioblastoma Patients of the Northeast of Morocco.
Author : Senhaji N1,Louati S1,Chbani L2,3,El Fatemi H2,3,Hammas N2,Mikou K1,Maaroufi M4,Benzagmout M5,Boujraf S6,7,El Bardai S2,Giry M8,Marie Y9,Chaoui El Faiz M5,Mokhtari K10,11,12,13,Idbaih A9,10,11,12,14,Amarti A2,3,Bennis S2,3.
Biomed Res Int. 2017;2017:8045859. doi: 10.1155/2017/8045859. Epub 2017 Jul 13.
28785587

BACKGROUND

INA is a class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NF-L to form the filamentous backbone to which NF-M and NF-H attach to form the cross-bridges.

REFERENCES

Armstrong,R.A.,Eur. J. Neurol. 13 (5), 528-532 (2006)
Suzuki,T., Eur. J. Neurosci. 21 (2), 339-350 (2005)
Cairns,N.J., Am. J. Pathol. 164 (6), 2153-2161 (2004)

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