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SOST Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - SOST Antibody (N-term) AP6261A
    Anti-SOST Antibody (N-term) at 1:2000 dilution + Recombinant human SOST protein lysate Lysates/proteins at 20 ng per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - SOST Antibody (N-term) AP6261A
    AP6261A staining SOST in Human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
  • 1 - SOST Antibody (N-term) AP6261A
    All lanes : Anti-SOST Antibody (N-term) at 1:1000 dilution Lane 1: human kidney lysates Lane 2: human liver lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - SOST Antibody (N-term) AP6261A
    Western blot analysis of lysates from Hela cell line, human lung, mouse lung, mouse liver tissue lysate (from left to right), using SOST Antibody (N-term)(Cat. #AP6261a). AP6261a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • 1 - SOST Antibody (N-term) AP6261A
    Western blot analysis of lysates from mouse liver, mouse lung, rat kidney tissue lysate (from left to right), using SOST Antibody (N-term)(Cat. #AP6261a). AP6261a was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.
  • 14 - SOST Antibody (N-term) AP6261A
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with SOST antibody (N-term)(Cat.#AP6261a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q9BQB4
Other Accession Q9BG79
Reactivity Human, Mouse, Rat
Predicted Bovine
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 50964
Other Names Sclerostin, SOST
Target/Specificity This SOST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human SOST.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSOST Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SOST
Function Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level)
Research Areas
Computational and functional characterization of four SNPs in the SOST locus associated with osteoporosis.
Author : Ye W1,Wang Y1,Mei B1,Hou S1,Liu X1,Wu G1,Qin L1,Zhao K1,Huang Q2.
Bone. 2018 Jan 4. pii: S8756-3282(18)30001-2. doi: 10.1016/j.bone.2018.01.001. [Epub ahead of print]
29307778
Co-expression of DKK-1 and Sclerostin in Subchondral Bone of the Proximal Femoral Heads from Osteoarthritic Hips.
Author : Zarei A1,Hulley PA1,Sabokbar A1,Javaid MK2.
Calcif Tissue Int. 2017 Mar 9. doi: 10.1007/s00223-017-0246-7. [Epub ahead of print]
28275825
Single-pulsed electromagnetic field therapy increases osteogenic differentiation through Wnt signaling pathway and sclerostin downregulation.
Author : Lin CC1,2, Lin RW1,3, Chang CW4, Wang GJ1,5,6, Lai KA1,4.
Bioelectromagnetics. 2015 Oct;36(7):494-505. doi: 10.1002/bem.21933. Epub 2015 Sep 14.
26364557

BACKGROUND

Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.

REFERENCES

Semenov,M.V., J. Biol. Chem. 281 (50), 38276-38284 (2006)
Ellies,D.L., J. Bone Miner. Res. 21 (11), 1738-1749 (2006)
Balemans,W., J Musculoskelet Neuronal Interact 6 (4), 355-356 (2006)
Gardner,J.C., J. Clin. Endocrinol. Metab. 90 (12), 6392-6395 (2005)

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