- 文献引用 : 3
|Application ||IHC-P-Leica, WB, E|
|Reactivity||Human, Mouse, Rat|
|Other Names||Sclerostin, SOST|
|Target/Specificity||This SOST antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 12-42 amino acids from the N-terminal region of human SOST.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||SOST Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation.|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
|Tissue Location||Widely expressed at low levels with highest levels in bone, cartilage, kidney, liver, bone marrow and primary osteoblasts differentiated for 21 days. Detected in the subendothelial layer of the aortic intima (at protein level)|
Author : Ye W1,Wang Y1,Mei B1,Hou S1,Liu X1,Wu G1,Qin L1,Zhao K1,Huang Q2.
Bone. 2018 Jan 4. pii: S8756-3282(18)30001-2. doi: 10.1016/j.bone.2018.01.001. [Epub ahead of print]
Author : Zarei A1,Hulley PA1,Sabokbar A1,Javaid MK2.
Calcif Tissue Int. 2017 Mar 9. doi: 10.1007/s00223-017-0246-7. [Epub ahead of print]
Author : Lin CC1,2, Lin RW1,3, Chang CW4, Wang GJ1,5,6, Lai KA1,4.
Bioelectromagnetics. 2015 Oct;36(7):494-505. doi: 10.1002/bem.21933. Epub 2015 Sep 14.
Provided below are standard protocols that you may find useful for product applications.
Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Loss-of-function mutations in this gene are associated with an autosomal-recessive disorder, sclerosteosis, which causes progressive bone overgrowth. A deletion downstream of this gene, which causes reduced sclerostin expression, is associated with a milder form of the disorder called van Buchem disease.
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