注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   细胞生物学   >   RAI17 Antibody (C-term)   

RAI17 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RAI17 Antibody (C-term) AP6236a
    All lanes : Anti-Rai17 Antibody (P604) at 1:2000 dilution Lane 1: K562 whole cell lysates Lane 2: 293T/17 whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 115 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - RAI17 Antibody (C-term) AP6236a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 产品详情
  • 文献引用 : 3
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession Q9ULJ6
Other Accession Q6P1E1
Reactivity Human
Predicted Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 115483 Da
Additional info
Gene ID 57178
Other Names Zinc finger MIZ domain-containing protein 1, PIAS-like protein Zimp10, Retinoic acid-induced protein 17, ZMIZ1, KIAA1224, RAI17, ZIMP10
Target/Specificity This RAI17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1038-1067 amino acids from the C-terminal region of human RAI17.
Dilution WB~~1:2000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsRAI17 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ZMIZ1
Synonyms KIAA1224, RAI17, ZIMP10
Function Acts as transcriptional coactivator. Increases ligand- dependent transcriptional activity of AR and promotes AR sumoylation. The stimulation of AR activity is dependent upon sumoylation (PubMed:14609956, PubMed:26522984). Involved in transcriptional activation of a subset of NOTCH1 target genes including MYC. Involved in thymocyte and T cell development (By similarity).
Cellular Location Nucleus speckle. Cytoplasm
Tissue Location Expressed most abundantly in ovary and, at lower levels, in prostate, spleen and testis. Weak expression, if any, in thymus, small intestine, colon and peripheral blood leukocytes.
Research Areas
Identification of Two Additional Susceptibility Loci for Inflammatory Bowel Disease in a Chinese Population.
Author : Lan X,Lan X,Chang Y,Zhang X,Liu J,Vikash V,Wang W,Huang M,Wang X,Zhou F,Chen L,Zhao Q.
Cell Physiol Biochem. 2017 Apr 14;41(5):2077-2090. doi: 10.1159/000475439. [Epub ahead of print]
28456797
The PIAS-like Coactivator Zmiz1 Is a Direct and Selective Cofactor of Notch1 in T Cell Development and Leukemia.
Author : Pinnell N1, Yan R2, Cho HJ3, Keeley T4, Murai MJ3, Liu Y2, Alarcon AS2, Qin J2, Wang Q2, Kuick R5, Elenitoba-Johnson KS6, Maillard I Immunity. 2015 Oct 27. pii: S1074-7613(15)00407-0. doi: 10.1016/j.immuni.2015.10.007. [Epub ahead of print]
26522984
Convergence of the ZMIZ1 and NOTCH1 pathways at C-MYC in acute T lymphoblastic leukemias.
Author : Rakowski LA1, Garagiola DD, Li CM, Decker M, Caruso S, Jones M, Kuick R, Cierpicki T, Maillard I, Chiang MY.
Cancer Res. 2013 Jan 15;73(2):930-41. doi: 10.1158/0008-5472.CAN-12-1389. Epub 2012 Nov 16.
23161489

BACKGROUND

Retinoic acid plays a critical role in development, cellular growth, and differentiation and induces the expression of a variety of genes. RAI17 expression is induced by retinoic acid and is predominantly expressed in heart, brain and ovaries. Within brain, highest expression is in amygdala. The deduced 1,067-amino acid protein contains an MSX-interacting zinc finger (MIZ) domain, a nuclear localization signal sequence, and 2 proline-rich regions. A strong intrinsic transactivation domain is identified in the C-terminal proline-rich region. RAI17 expression is detected in various cancer cell lines. RAI17 colocalizes with endogenous androgen receptor (AR) in the nuclei of prostate epithelial cells from human tissue samples. In human prostate cancer cells, RAI17 increases the transcriptional activity of AR. Studies using sumoylation-deficient AR mutants suggest that the increase of AR activity by RAI17 is dependent upon receptor sumoylation.

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价