- 文献引用 : 7
|Application ||WB, IHC-P, E|
|Calculated MW||46029 Da|
|Other Names||2'-5'-oligoadenylate synthase 1, (2-5')oligo(A) synthase 1, 2-5A synthase 1, E18/E16, p46/p42 OAS, OAS1, OIAS|
|Target/Specificity||This OAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the C-terminal region of human OAS1.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||OAS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L.|
|Cellular Location||Cytoplasm Mitochondrion. Nucleus Microsome. Endoplasmic reticulum. Secreted Note=Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions|
Author : Gielen V, Johnston SL, Edwards MR.
Eur Respir J. 2010 Sep;36(3):646-54. doi: 10.1183/09031936.00095809. Epub 2010 Feb 11.
Author : Kriegs M, BÃ¼rckstÃ¼mmer T, Himmelsbach K, Bruns M, Frelin L, AhlÃ©n G, SÃ¤llberg M, Hildt E.
J Biol Chem. 2009 Oct 9;284(41):28343-51. doi: 10.1074/jbc.M109.038877. Epub 2009 Aug 12.
Author : Wang H, Ghosh A, Baigude H, Yang CS, Qiu L, Xia X, Zhou H, Rana TM, Xu Z.
J Biol Chem. 2008 Jun 6;283(23):15845-52. doi: 10.1074/jbc.M800834200. Epub 2008 Mar 26.
Author : Han JQ, Townsend HL, Jha BK, Paranjape JM, Silverman RH, Barton DJ.
J Virol. 2007 Jun;81(11):5561-72. Epub 2007 Mar 7.
Author : Alimirah F, Chen J, Davis FJ, Choubey D.
Mol Cancer Res. 2007 Mar;5(3):251-9. Epub 2007 Mar 5.
Author : van den Pol AN, Robek MD, Ghosh PK, Ozduman K, Bandi P, Whim MD, Wollmann G.
J Virol. 2007 Jan;81(1):332-48. Epub 2006 Oct 25.
Author : Lee J, Wu CC, Lee KJ, Chuang TH, Katakura K, Liu YT, Chan M, Tawatao R, Chung M, Shen C, Cottam HB, Lai MM, Raz E, Carson DA.
Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1828-33. Epub 2006 Jan 30.
Provided below are standard protocols that you may find useful for product applications.
OAS1 is an interferon inducible protein that may play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis. It binds double-stranded RNA and polymerizes ATP into PPP(A2'P5'A)N oligomers, which activate the latent RNase L that, when activated, cleaves single-stranded RNAs. This protein is associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions.
Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Sarkar, S.N., et al., J. Biol. Chem. 274(36):25535-25542 (1999).
Ghosh, A., et al., J. Biol. Chem. 272(52):33220-33226 (1997).
Ghosh, S.K., et al., J. Biol. Chem. 266(23):15293-15299 (1991).
Rutherford, M.N., et al., EMBO J. 7(3):751-759 (1988).