注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   其他   >   OAS1 Antibody (C-term)   

OAS1 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - OAS1 Antibody (C-term) AP6226a
    All lanes : Anti-OAS1 Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Human kidney lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 44 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - OAS1 Antibody (C-term) AP6226a
    The anti-OAS1 Pab (Cat. #AP6226a) is used in Western blot to detect OAS1 in mouse liver lysate.
  • 14 - OAS1 Antibody (C-term) AP6226a
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with OAS1 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 产品详情
  • 文献引用 : 7
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P00973
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Calculated MW 46029 Da
Additional info
Gene ID 4938
Other Names 2'-5'-oligoadenylate synthase 1, (2-5')oligo(A) synthase 1, 2-5A synthase 1, E18/E16, p46/p42 OAS, OAS1, OIAS
Target/Specificity This OAS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 302-330 amino acids from the C-terminal region of human OAS1.
Dilution WB~~1:1000
IHC-P~~1:10~50
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsOAS1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name OAS1
Synonyms OIAS
Function Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L.
Cellular Location Cytoplasm. Mitochondrion. Nucleus. Microsome. Endoplasmic reticulum. Secreted Note=Associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions
Research Areas
Azithromycin induces anti-viral responses in bronchial epithelial cells.
Author : Gielen V, Johnston SL, Edwards MR.
Eur Respir J. 2010 Sep;36(3):646-54. doi: 10.1183/09031936.00095809. Epub 2010 Feb 11.
20150207
The hepatitis C virus non-structural NS5A protein impairs both the innate and adaptive hepatic immune response in vivo.
Author : Kriegs M, Bürckstümmer T, Himmelsbach K, Bruns M, Frelin L, Ahlén G, Sällberg M, Hildt E.
J Biol Chem. 2009 Oct 9;284(41):28343-51. doi: 10.1074/jbc.M109.038877. Epub 2009 Aug 12.
19674968
Therapeutic gene silencing delivered by a chemically modified small interfering RNA against mutant SOD1 slows amyotrophic lateral sclerosis progression.
Author : Wang H, Ghosh A, Baigude H, Yang CS, Qiu L, Xia X, Zhou H, Rana TM, Xu Z.
J Biol Chem. 2008 Jun 6;283(23):15845-52. doi: 10.1074/jbc.M800834200. Epub 2008 Mar 26.
18367449
A phylogenetically conserved RNA structure in the poliovirus open reading frame inhibits the antiviral endoribonuclease RNase L.
Author : Han JQ, Townsend HL, Jha BK, Paranjape JM, Silverman RH, Barton DJ.
J Virol. 2007 Jun;81(11):5561-72. Epub 2007 Mar 7.
17344297
IFI16 in human prostate cancer.
Author : Alimirah F, Chen J, Davis FJ, Choubey D.
Mol Cancer Res. 2007 Mar;5(3):251-9. Epub 2007 Mar 5.
17339605
Cytomegalovirus induces interferon-stimulated gene expression and is attenuated by interferon in the developing brain.
Author : van den Pol AN, Robek MD, Ghosh PK, Ozduman K, Bandi P, Whim MD, Wollmann G.
J Virol. 2007 Jan;81(1):332-48. Epub 2006 Oct 25.
17065212
Activation of anti-hepatitis C virus responses via Toll-like receptor 7.
Author : Lee J, Wu CC, Lee KJ, Chuang TH, Katakura K, Liu YT, Chan M, Tawatao R, Chung M, Shen C, Cottam HB, Lai MM, Raz E, Carson DA.
Proc Natl Acad Sci U S A. 2006 Feb 7;103(6):1828-33. Epub 2006 Jan 30.
16446426

BACKGROUND

OAS1 is an interferon inducible protein that may play a role in mediating resistance to virus infection, control of cell growth, differentiation, and apoptosis. It binds double-stranded RNA and polymerizes ATP into PPP(A2'P5'A)N oligomers, which activate the latent RNase L that, when activated, cleaves single-stranded RNAs. This protein is associated with different subcellular fractions such as mitochondrial, nuclear, and rough/smooth microsomal fractions.

REFERENCES

Strausberg, R.L., et al., Proc. Natl. Acad. Sci. U.S.A. 99(26):16899-16903 (2002).
Sarkar, S.N., et al., J. Biol. Chem. 274(36):25535-25542 (1999).
Ghosh, A., et al., J. Biol. Chem. 272(52):33220-33226 (1997).
Ghosh, S.K., et al., J. Biol. Chem. 266(23):15293-15299 (1991).
Rutherford, M.N., et al., EMBO J. 7(3):751-759 (1988).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价