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MMP9 Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - MMP9 Antibody (C-term) AP6214a
    All lanes : Anti-MMP9 Antibody (L659) at 1:2000 dilution Lane 1: NCI-H1299 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: A431 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 14 - MMP9 Antibody (C-term) AP6214a
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
  • 14 - MMP9 Antibody (C-term) AP6214a
    Formalin-fixed and paraffin-embedded human Spleen tissue reacted with MMP9 antibody (C-term) (Cat.#AP6214a), which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
  • 14 - MMP9 Antibody (C-term) AP6214a
    Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with MMP9 antibody (C-term) (Cat.#AP6214a), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
IHC-P, WB, E
Primary Accession P14780
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 4318
Other Names Matrix metalloproteinase-9, MMP-9, 92 kDa gelatinase, 92 kDa type IV collagenase, Gelatinase B, GELB, 67 kDa matrix metalloproteinase-9, 82 kDa matrix metalloproteinase-9, MMP9, CLG4B
Target/Specificity This MMP9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 644-673 amino acids from the C-terminal region of human MMP9.
Dilution WB~~1:2000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsMMP9 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name MMP9
Synonyms CLG4B
Function May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Cellular Location Secreted, extracellular space, extracellular matrix
Tissue Location Detected in neutrophils (at protein level) (PubMed:7683678). Produced by normal alveolar macrophages and granulocytes.
Research Areas
Effects of secreted frizzled-related protein 1 on proliferation, migration, invasion, and apoptosis of colorectal cancer cells.
Author : Wang Z#1,Li R#1,He Y1,Huang S1.
Cancer Cell Int. 2018 Mar 27;18:48. doi: 10.1186/s12935-018-0543-x. eCollection 2018.
29610564
Association of MMP-2 and MMP-9 expression with recurrences in primary spontaneous pneumothorax.
Author : Huang YF1,Chiu WC2,Chou SH2,Su YH3,Chen YW1,Chai CY4,Huang CJ5,Huang MY5,Yuan SF6,Lee YC7.
Kaohsiung J Med Sci. 2017 Jan;33(1):17-23. doi: 10.1016/j.kjms.2016.10.007. Epub 2016 Nov 12.
28088269
Nkx2-5 Is Expressed in Atherosclerotic Plaques and Attenuates Development of Atherosclerosis in Apolipoprotein E-Deficient Mice.
Author : Du M1,2,Wang X1,2,Tan X1,2,Li X1,2,Huang D1,2,Huang K1,2,Yang L1,2,Zhang F1,2,Wang Y1,2,Huang D1,2,Huang K3,2.
J Am Heart Assoc. 2016 Dec 19;5(12). pii: e004440.
27993833
EFEMP1 inhibits migration of hepatocellular carcinoma by regulating MMP2 and MMP9 via ERK1/2 activity.
Author : Dou CY1, Cao CJ2, Wang Z2, Zhang RH3, Huang LL3, Lian JY2, Xie WL2, Wang LT2.
Oncol Rep. 2016 Apr 5. doi: 10.3892/or.2016.4733. [Epub ahead of print]
27108677
Ethanol-Induced TLR4/NLRP3 Neuroinflammatory Response in Microglial Cells Promotes Leukocyte Infiltration Across the BBB.
Author : Alfonso-Loeches S1, Ureña-Peralta J1, Morillo-Bargues MJ1, Gómez-Pinedo U2, Guerri C3.
Neurochem Res. 2015 Nov 11. [Epub ahead of print]
26555554
Down-regulation of TCF21 by hypermethylation induces cell proliferation, migration and invasion in colorectal cancer.
Author : Dai Y1, Duan H2, Duan C3, Zhou R1, He Y1, Tu Q1, Shen L4.
Biochem Biophys Res Commun. 2015 Oct 1. pii: S0006-291X(15)30626-4. doi: 10.1016/j.bbrc.2015.09.109. [Epub ahead of print]
26435499
Calreticulin promotes migration and invasion of esophageal cancer cells by upregulating neuropilin-1 expression via STAT5A.
Author : Shi F1, Shang L1, Pan BQ1, Wang XM1, Jiang YY1, Hao JJ1, Zhang Y1, Cai Y1, Xu X1, Zhan QM1, Wang MR2.
Clin Cancer Res. 2014 Dec 1;20(23):6153-62. doi: 10.1158/1078-0432.CCR-14-0583. Epub 2014 Sep 17.
25231404
High expression of Dickkopf-related protein 1 is related to lymphatic metastasis and indicates poor prognosis in intrahepatic cholangiocarcinoma patients after surgery.
Author : Shi RY, Yang XR, Shen QJ, Yang LX, Xu Y, Qiu SJ, Sun YF, Zhang X, Wang Z, Zhu K, Qin WX, Tang ZY, Fan J, Zhou J.
Cancer. 2013 Mar 1;119(5):993-1003. doi: 10.1002/cncr.27788. Epub 2012 Nov 6.
23132676
A lipoxin A4 analog ameliorates blood-brain barrier dysfunction and reduces MMP-9 expression in a rat model of focal cerebral ischemia-reperfusion injury.
Author : Wu Y, Wang YP, Guo P, Ye XH, Wang J, Yuan SY, Yao SL, Shang Y.
J Mol Neurosci. 2012 Mar;46(3):483-91. doi: 10.1007/s12031-011-9620-5. Epub 2011 Aug 16.
21845429
OxLDL up-regulates microRNA-29b, leading to epigenetic modifications of MMP-2/MMP-9 genes: a novel mechanism for cardiovascular diseases.
Author : Chen KC1, Wang YS, Hu CY, Chang WC, Liao YC, Dai CY, Juo SH.
FASEB J. 2011 May;25(5):1718-28. doi: 10.1096/fj.10-174904. Epub 2011 Jan 25.
21266537

BACKGROUND

Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP9 degrades type IV and V collagens. Studies in rhesus monkeys suggest that the enzyme is involved in IL-8-induced mobilization of hematopoietic progenitor cells from bone marrow, and murine studies suggest a role in tumor-associated tissue remodeling.

REFERENCES

Tutton, M.G., et al., Int. J. Cancer 107(4):541-550 (2003). Behrens, P., et al., Int. J. Cancer 107(2):183-188 (2003). Van den Steen, P.E., et al., Biochem. Biophys. Res. Commun. 310(3):889-896 (2003). Zhao, Z., et al., J. Infect. Dis. 188(8):1098-1104 (2003). Matsuyama, A., et al., Circulation 108(12):1469-1473 (2003).

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