|Application ||IHC-P, FC, WB, E|
|Calculated MW||53977 Da|
|Other Names||Stromelysin-1, SL-1, Matrix metalloproteinase-3, MMP-3, Transin-1, MMP3, STMY1|
|Target/Specificity||This MMP3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 298-327 amino acids from the Central region of human MMP3.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MMP3 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.|
|Cellular Location||Secreted, extracellular space, extracellular matrix|
Provided below are standard protocols that you may find useful for product applications.
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. MMP3 is an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation.
Sage, E.H., et al., J. Biol. Chem. 278(39):37849-37857 (2003).
Matsuyama, A., et al., Circulation 108(12):1469-1473 (2003).
Mercapide, J., et al., Int. J. Cancer 106(5):676-682 (2003).
Bodemer, C., et al., J. Invest. Dermatol. 121(2):273-279 (2003).
Kang, M.K., et al., Exp. Cell Res. 287(2):272-281 (2003).