注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   信号转导   >   GREMLIN Antibody (C-term)   

GREMLIN Antibody (C-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - GREMLIN Antibody (C-term) AP6133a
    Anti-GREMLIN Antibody (C-term) at 1:2000 dilution + Mouse skeletal muscle lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 21 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 文献引用 : 10
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, E
Primary Accession O70326
Other Accession O35793, O60565
Reactivity Human, Mouse
Predicted Rat
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 23892
Other Names Gremlin-1, Cysteine knot superfamily 1, BMP antagonist 1, Down-regulated in Mos-transformed cells protein, Grem1, Cktsf1b1, Drm
Target/Specificity This GREMLIN antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 147-175 amino acids from the C-terminal region of human GREMLIN.
Dilution WB~~1:2000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsGREMLIN Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name Grem1
Synonyms Cktsf1b1, Drm
Function Cytokine that may play an important role during carcinogenesis and metanephric kidney organogenesis, as BMP a antagonist required for early limb outgrowth and patterning in maintaining the FGF4-SHH feedback loop. Down-regulates the BMP4 signaling in a dose-dependent manner. Acts as inhibitor of monocyte chemotaxis.
Cellular Location Secreted.
Tissue Location Highly expressed in spleen and to a lesser extent in lung, skeletal muscle and kidney. Expressed only in non- transformed cells or primary fibroblasts in culture but not in established transformed or tumor derived cell lines. Broadly expressed in limb bud mesenchyme but restricted to the distal limb bud mesenchyme and concentrated posteriorly. Expressed in ovary especially in granulosa cells of follicles of type 4
Research Areas
Gremlin activates the Notch pathway linked to renal inflammation.
Author : Lavoz C1,Poveda J2,Márquez-Expósito L3,Rayego-Mateos S2,Rodrigues-Diez RR4,Ortiz A5,Egido J3,Mezzano S1,Ruiz-Ortega M6.
Clin Sci (Lond). 2018 May 2. pii: CS20171553. doi: 10.1042/CS20171553. [Epub ahead of print]
29720422
Gremlin is a key pro-fibrogenic factor in chronic pancreatitis.
Author : Staloch D1, Gao X, Liu K, Xu M, Feng X, Aronson JF, Falzon M, Greeley GH, Rastellini C, Chao C, Hellmich MR, Cao Y, Ko TC.
J Mol Med (Berl). 2015 Jul 5. [Epub ahead of print]
26141517
Tubular overexpression of gremlin induces renal damage susceptibility in mice.
Author : Droguett A1, Krall P1, Burgos ME1, Valderrama G1, Carpio D2, Ardiles L1, Rodriguez-Diez R3, Kerr B4, Walz K4, Ruiz-Ortega M3, Egido J3
PLoS One. 2014 Jul 18;9(7):e101879. doi: 10.1371/journal.pone.0101879. eCollection 2014.
25036148
Identification of target genes of PAX3-FOXO1 in alveolar rhabdomyosarcoma.
Author : Ahn EH, Mercado GE, Laé M, Ladanyi M.
Oncol Rep. 2013 Aug;30(2):968-78. doi: 10.3892/or.2013.2513. Epub 2013 Jun 3.
23733015
Molecular differentiation between osteophytic and articular cartilage--clues for a transient and permanent chondrocyte phenotype.
Author : Gelse K, Ekici AB, Cipa F, Swoboda B, Carl HD, Olk A, Hennig FF, Klinger P.
Osteoarthritis Cartilage. 2012 Feb;20(2):162-71. doi: 10.1016/j.joca.2011.12.004. Epub 2011 Dec 13.
22209871
Expression of gremlin, a bone morphogenetic protein antagonist,is associated with vascular calcification in uraemia.
Author : Jara A, Chacón C, Burgos ME, Droguett A, Valdivieso A, Ortiz M, Troncoso P, Mezzano S.
Nephrol Dial Transplant. 2009 Apr;24(4):1121-9. doi: 10.1093/ndt/gfn611. Epub 2008 Nov 21.
19028757
Gremlin: a novel mediator of epithelial mesenchymal transition and fibrosis in chronic allograft nephropathy.
Author : Carvajal G, Droguett A, Burgos ME, Aros C, Ardiles L, Flores C, Carpio D, Ruiz-Ortega M, Egido J, Mezzano S.
Transplant Proc. 2008 Apr;40(3):734-9. doi: 10.1016/j.transproceed.2008.02.064.
18455002
Expression of gremlin, a bone morphogenetic protein antagonist, in glomerular crescents of pauci-immune glomerulonephritis.
Author : Mezzano S, Droguett A, Burgos ME, Aros C, Ardiles L, Flores C, Carpio D, Carvajal G, Ruiz-Ortega M, Egido J.
Nephrol Dial Transplant. 2007 Jul;22(7):1882-90. Epub 2007 Apr 1.
17403698
Effects of TGF-beta2, BMP-4, and gremlin in the trabecular meshwork: implications for glaucoma.
Author : Wordinger RJ, Fleenor DL, Hellberg PE, Pang IH, Tovar TO, Zode GS, Fuller JA, Clark AF.
Invest Ophthalmol Vis Sci. 2007 Mar;48(3):1191-200.
17325163
Regulation of myogenic progenitor proliferation in human fetal skeletal muscle by BMP4 and its antagonist Gremlin.
Author : Frank NY, Kho AT, Schatton T, Murphy GF, Molloy MJ, Zhan Q, Ramoni MF, Frank MH, Kohane IS, Gussoni E.
J Cell Biol. 2006 Oct 9;175(1):99-110. Epub 2006 Oct 2.
17015616

BACKGROUND

GREMLIN is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to relay the sonic hedgehog (SHH) signal from the polarizing region to the apical ectodermal ridge during limb bud outgrowth.

REFERENCES

Chen, B., et al., J. Immunol. 173(10):5914-5917 (2004). Chen, B., et al., Biochem. Biophys. Res. Commun. 295(5):1135-1141 (2002). McMahon, R., et al., J. Biol. Chem. 275(14):9901-9904 (2000). Murphy, M., et al., J. Biol. Chem. 274(9):5830-5834 (1999). Hsu, D.R., et al., Mol. Cell 1(5):673-683 (1998).

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价