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ABCB5 Antibody (N-term)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - ABCB5 Antibody (N-term) AP6122a
    Overlay histogram showing HepG2 cells stained with AP6122a(green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP6122a, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - ABCB5 Antibody (N-term) AP6122a
    All lanes : Anti-ABCB5 Antibody (N-term) at 1:2000 dilution Lane 1: A375 whole cell lysate Lane 2: HL-60 whole cell lysate Lane 3: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 138 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ABCB5 Antibody (N-term) AP6122a
    Anti-ABCB5 Antibody (N-term) at 1:2000 dilution + Mouse eyeball lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 138 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ABCB5 Antibody (N-term) AP6122a
    All lanes : Anti-ABCB5 Antibody (N-term) at 1:1000 dilution Lane 1: A431 whole cell lysate Lane 2: K562 whole cell lysate Lane 3: HL-60 whole cell lysate Lane 4: A375 whole cell lysate Lane 5: A2058 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 138 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q2M3G0
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 340273
Other Names ATP-binding cassette sub-family B member 5, ABCB5 P-gp, P-glycoprotein ABCB5, ABCB5
Target/Specificity This ABCB5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human ABCB5.
Dilution FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABCB5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABCB5
Function Drug efflux transporter present in a number of stem cells that acts as a regulator of cellular differentiation. Able to mediate efflux from cells of the rhodamine dye and of the therapeutic drug doxorubicin. Specifically present in limbal stem cells, where it plays a key role in corneal development and repair.
Cellular Location Cell membrane; Multi-pass membrane protein {ECO:0000255|PROSITE-ProRule:PRU00441, ECO:0000269|PubMed:12960149}
Tissue Location Expressed by CD133-expressing progenitor cells among epidermal melanocytes (at protein level). Widely expressed with specific expression in pigment cells. Highly expressed in several malignant tissues: highly expressed in clinical melanomas, with low expression in normal skin. In melanoma, marks malignant melanoma-initiating cells (MMIC), in which clinical virulence resides as a consequence of unlimited self-renewal capacity, resulting in inexorable tumor progression and metastasis. Also highly expressed in a number of leukemia cells. Expressed in basal limbal epithelium.
Research Areas
Side population cells from human melanoma tumors reveal diverse mechanisms for chemoresistance.
Author : Luo Y1, Ellis LZ, Dallaglio K, Takeda M, Robinson WA, Robinson SE, Liu W, Lewis KD, McCarter MD, Gonzalez R, Norris DA, Roop DR, Spritz RA, Ahn NG, Fujita M.
J Invest Dermatol. 2012 Oct;132(10):2440-50. doi: 10.1038/jid.2012.161. Epub 2012 May 24.
22622430
CD133+ melanoma subpopulations contribute to perivascular niche morphogenesis and tumorigenicity through vasculogenic mimicry.
Author : Lai CY1, Schwartz BE, Hsu MY.
Cancer Res. 2012 Oct 1;72(19):5111-8. doi: 10.1158/0008-5472.CAN-12-0624. Epub 2012 Aug 3.
22865455
Evaluation of a multi-marker immunomagnetic enrichment assay for the quantification of circulating melanoma cells.
Author : Freeman JB1, Gray ES, Millward M, Pearce R, Ziman M.
J Transl Med. 2012 Sep 15;10:192. doi: 10.1186/1479-5876-10-192.
22978632
ABCB5 identifies a therapy-refractory tumor cell population in colorectal cancer patients.
Author : Wilson BJ, Schatton T, Zhan Q, Gasser M, Ma J, Saab KR, Schanche R, Waaga-Gasser AM, Gold JS, Huang Q, Murphy GF, Frank MH, Frank NY.
Cancer Res. 2011 Aug 1;71(15):5307-16. doi: 10.1158/0008-5472.CAN-11-0221. Epub 2011 Jun 7.
21652540
Melanoma spheroids grown under neural crest cell conditions are highly plastic migratory/invasive tumor cells endowed with immunomodulator function.
Author : Ramgolam K, Lauriol J, Lalou C, Lauden L, Michel L, de la Grange P, Khatib AM, Aoudjit F, Charron D, Alcaide-Loridan C, Al-Daccak R.
PLoS One. 2011 Apr 15;6(4):e18784. doi: 10.1371/journal.pone.0018784.
21526207

BACKGROUND

ABCB5 belongs to the ATP-binding cassette (ABC) transporter superfamily of integral membrane proteins. These proteins participate in ATP-dependent transmembrane transport of structurally diverse molecules ranging from small ions, sugars, and peptides to more complex organic molecules.

REFERENCES

Frank,N.Y., Cancer Res. 65 (10), 4320-4333 (2005) Chen,K.G., Pigment Cell Res. 18 (2), 102-112 (2005) Frank,N.Y., J. Biol. Chem. 278 (47), 47156-47165 (2003)

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