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ABCB4 Antibody (Center)

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - ABCB4 Antibody (Center) AP6112A
    All lanes : Anti-hABCB4-S639 at 1:500 dilution Lane 1: HepG2 whole cell lysate Lane 2: U-2OS whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 142 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ABCB4 Antibody (Center) AP6112A
    Anti-hABCB4-S639 at 1:2000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 142 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - ABCB4 Antibody (Center) AP6112A
    ABCB4-S639 (Cat. #AP6112a) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the ABCB4 antibody detected ABCB4 protein (arrow).
  • 14 - ABCB4 Antibody (Center) AP6112A
    Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession P21439
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 5244
Other Names Multidrug resistance protein 3, ATP-binding cassette sub-family B member 4, P-glycoprotein 3, ABCB4, MDR3, PGY3
Target/Specificity This ABCB4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 624-654 amino acids from the Central region of human ABCB4.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsABCB4 Antibody (Center) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name ABCB4
Synonyms MDR3, PGY3
Function Mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte.
Cellular Location Cell membrane; Multi-pass membrane protein.
Research Areas
Hepatic lipid accumulation in apolipoprotein C-I-deficient mice is potentiated by cholesteryl ester transfer protein.
Author : Gautier T, Tietge UJ, Boverhof R, Perton FG, Le Guern N, Masson D, Rensen PC, Havekes LM, Lagrost L, Kuipers F.
J Lipid Res. 2007 Jan;48(1):30-40. Epub 2006 Oct 19.
17053273

BACKGROUND

The membrane-associated protein encoded ABCB4 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function.

REFERENCES

Eloranta, M.L., et al., Eur J Obstet Gynecol Reprod Biol 105(2):132-135 (2002).
Eloranta, M.L., et al., Eur J Obstet Gynecol Reprod Biol 104(2):109-112 (2002).
Jacquemin, E., Semin. Liver Dis. 21(4):551-562 (2001).
Smit, J.J., et al., Biochim. Biophys. Acta 1261(1):44-56 (1995).
Ruetz, S., et al., Cell 77(7):1071-1081 (1994).

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