KIAA1688 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Overlay histogram showing Hela cells stained with AP5966b(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP5966b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
All lanes : Anti-KIAA1688 Antibody (C-term) at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: MOLT-4 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: Mouse brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 121 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Product info
Application Applications Legend: E=ELISA WB=Western Blotting IHC=Immunohistochemistry IHC-P=Immunohistochemistry (Paraffin) IP=Immunoprecipitation IF=Immunofluorescence IC=Immunochemistry ICC=Immunocytochemistry FC=Flow Cytometry DB=Dot Blot	FC, WB, E
Primary Accession	Q9C0H5
Other Accession	P59281, NP_079527.1
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit Ig

Additional info
Gene ID	80728
Other Names	Rho GTPase-activating protein 39, ARHGAP39, KIAA1688
Target/Specificity	This KIAA1688 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 852-880 amino acids from the C-terminal region of human KIAA1688.
Dilution	FC~~1:25 WB~~1:2000
Format	Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Precautions	KIAA1688 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.

Research Areas

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¥ 2,310.00

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Cat# AP5966b

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