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SLC10A1 Antibody (C-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
  • 4 - SLC10A1 Antibody (C-term) AP5710B
    Overlay histogram showing HepG2 cells stained with AP5710b(green line). The cells were fixed with 2% paraformaldehyde 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP5710b, 1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(1583138) at 1/200 dilution for 40 min at 37ºC. Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.
  • 1 - SLC10A1 Antibody (C-term) AP5710B
    All lanes : Anti-SLC10A1 Antibody (C-term) at 1:1000 dilution Lane 1: HT-1080 whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: K562 whole cell lysate Lane 4: Caco2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 38 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
FC, WB, E
Primary Accession Q14973
Other Accession NP_003040.1
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 6554
Other Names Sodium/bile acid cotransporter, Cell growth-inhibiting gene 29 protein, Na(+)/bile acid cotransporter, Na(+)/taurocholate transport protein, Sodium/taurocholate cotransporting polypeptide, Solute carrier family 10 member 1, SLC10A1, NTCP
Target/Specificity This SLC10A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 316-343 amino acids from the C-terminal region of human SLC10A1.
Dilution FC~~1:25
WB~~1:1000
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsSLC10A1 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name SLC10A1
Synonyms NTCP
Function The hepatic sodium/bile acid uptake system exhibits broad substrate specificity and transports various non-bile acid organic compounds as well. It is strictly dependent on the extracellular presence of sodium.
Cellular Location Membrane; Multi-pass membrane protein.
Research Areas
Core-fucosylation plays a pivotal role in hepatitis B pseudo virus infection: a possible implication for HBV glycotherapy.
Author : Takamatsu S1,Shimomura M1,Kamada Y2,Maeda H1,Sobajima T1,Hikita H3,Iijima M4,Okamoto Y5,Misaki R5,Fujiyama K5,Nagamori S6,Kanai Y6,Takehara T3,Ueda K7,Kuroda S4,Miyoshi E8.
Glycobiology. 2016 Jun 21. [Epub ahead of print]
27329181

BACKGROUND

Sodium/bile acid cotransporters are integral membrane glycoproteins that participate in the enterohepatic circulation of bile acids. Two homologous transporters are involved in the reabsorption of bile acids, one absorbing from the intestinal lumen, the bile duct, and the kidney with an apical localization (SLC10A2; MIM 601295), and the other being found in the basolateral membranes of hepatocytes (SLC10A1).

REFERENCES

Ho, R.H., et al. J. Biol. Chem. 279(8):7213-7222(2004)
Trauner, M., et al. Physiol. Rev. 83(2):633-671(2003)
Hallen, S., et al. Biochemistry 41(23):7253-7266(2002)
Shiao, T., et al. Genomics 69(2):203-213(2000)
Hagenbuch, B., et al. J. Clin. Invest. 93(3):1326-1331(1994)

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