|Application ||IHC-P-Leica, WB, E|
|Other Accession||Q6GM44, Q3KNM2, Q5ZJ41, Q3ZC24, NP_060294|
|Predicted||Chicken, Xenopus, Bovine|
|Other Names||E3 ubiquitin-protein ligase MARCH5, 632-, Membrane-associated RING finger protein 5, Membrane-associated RING-CH protein V, MARCH-V, Mitochondrial ubiquitin ligase, MITOL, RING finger protein 153, MARCH5, RNF153|
|Target/Specificity||This MARCH5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-32 amino acids from the N-terminal region of human MARCH5.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||MARCH5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.|
|Cellular Location||Mitochondrion outer membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Note=Authors show that the protein can be detected in endoplasmic reticulum (PubMed:14722266). Authors (PubMed:16874301) show its presence only in mitochondria (PubMed:16874301).|
|Tissue Location||Expressed in brain, heart, liver, lung, spleen, stomach, testis, skeletal and muscle|
Provided below are standard protocols that you may find useful for product applications.
MARCH5 is a ubiquitin ligase of the mitochondrial outer membrane that plays a role in the control of mitochondrial morphology by regulating mitofusin-2 (MFN2; MIM 608507) and DRP1 (DNM1L; MIM 603850) (Nakamura et al., 2006 [PubMed 16936636]).
Yonashiro, R., et al. Mol. Biol. Cell 20(21):4524-4530(2009) Karbowski, M., et al. J. Cell Biol. 178(1):71-84(2007) Nakamura, N., et al. EMBO Rep. 7(10):1019-1022(2006)