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DEPDC1 Antibody (N-term)

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

     
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  • 1 - DEPDC1 Antibody (N-term) AP5428a
    All lanes : Anti-DEPDC1 Antibody (N-term) at 1:1000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: A431 whole cell lysate Lysates/proteins at 30 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 93kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 1 - DEPDC1 Antibody (N-term) AP5428a
    DEPDC1 Antibody (N-term)(Cat. #AP5428a) western blot analysis in U251 cell line lysates (35ug/lane).This demonstrates the DEPDC1 antibody detected the DEPDC1 protein (arrow).
  • 14 - DEPDC1 Antibody (N-term) AP5428a
    DEPDC1 Antibody (N-term) (Cat. #AP5428a) immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the DEPDC1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC-P, E
Primary Accession Q5TB30
Other Accession NP_001107592.1, NP_060249.2
Reactivity Human
Host Rabbit
Clonality Polyclonal
Isotype Rabbit Ig
Additional info
Gene ID 55635
Other Names DEP domain-containing protein 1A, DEPDC1, DEPDC1A
Target/Specificity This DEPDC1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 10-38 amino acids from the N-terminal region of human DEPDC1.
Dilution WB~~1:1000
IHC-P~~1:50~100
Format Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
PrecautionsDEPDC1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
Protein Information
Name DEPDC1
Synonyms DEPDC1A
Function May be involved in transcriptional regulation as a transcriptional corepressor. The DEPDC1A-ZNF224 complex may play a critical role in bladder carcinogenesis by repressing the transcription of the A20 gene, leading to transport of NF-KB protein into the nucleus, resulting in suppression of apoptosis of bladder cancer cells.
Cellular Location Nucleus. Note=Colocalizes with ZNF224 at the nucleus
Tissue Location Expressed in testis. Up-regulated in bladder cancer cells (at protein level).
Research Areas

REFERENCES

Kanehira, M., et al. Oncogene 26(44):6448-6455(2007)

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