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Anti-eIF4E Antibody

Mouse Monoclonal Antibody

     
  • 1 - Anti-eIF4E Antibody AP53474
    Western blot detection of eIF4E in MDA-MB-468, ZR75-1, MCF7, T47D and CHO-K1 cell lysates using eIF4E mouse mAb (1:1000 diluted). Predicted band size: 25KDa. Observed band size:25KDa.
  • 1 - Anti-eIF4E Antibody AP53474
    Western blot detection of eIF4E in Jurkat, Hela, MCF7, K562, C6, 3T3, PC12, HepG2, CHO-K1, Raji, Raw264.7 and Ramos cell lysates using eIF4E mouse mAb (1:2000 diluted). Predicted band size: 25KDa. Observed band size:25KDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P06730
Other Accession NM_001968
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype lgG1
Immunogen Purified recombinant human eif4e protein expressed in E.coli.
Purification Affinity purified
Calculated MW 25kDa KDa
Additional Information
Gene ID 1977
Other Names AUTS19; CBP; eIF 4E; eIF 4F 25 kDa subunit; EIF 4F; eIF-4E; eIF-4F 25 kDa subunit; eIF4E; EIF4E1; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4 E; Eukaryotic translation initiation factor 4E; Eukaryotic translation initiation factor 4E like 1; IF4E_HUMAN; Messanger RNA Cap Binding Protein eIF 4E; MGC111573; mRNA cap binding protein; mRNA cap-binding protein.
Dilution WB~~1:1000
Format Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name EIF4E
Synonyms EIF4EL1, EIF4F
Function Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
Cellular Location Cytoplasm, P-body. Cytoplasm
Research Areas

BACKGROUND

Its translation stimulation activity is repressed by binding to the complex CYFIP1-FMR1 (By similarity). Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome bi

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