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Anti-CDK4 Antibody

Mouse Monoclonal Antibody

     
  • 1 - Anti-CDK4 Antibody AP53463
    Western blot detection of CDK4 in Hela and K562 cell lysates using CDK4 mouse mAb (1:500 diluted).Predicted band size:34KDa.Observed band size:34KDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P11802
Other Accession NM_000075.2
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype lgG1
Immunogen Purified recombinant human CDK4 protein expressed in E.coli.
Purification Affinity purified
Calculated MW 34kDa KDa
Additional Information
Gene ID 1019
Other Names Cdk4;cdk4;CDK4protein;CDK4_HUMAN;Celldivisionkinase4;Celldivisionproteinkinase4;CMM3;CMM3;Crk3;Cyclindependentkinase4;Cyclin-dependentkinase4;Melanomacutaneousmalignant3;MGC14458;p34cdk4;PSKJ3;PSK-J3.
Dilution WB~~1:500
Format Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CDK4
Function Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase. Hypophosphorylates RB1 in early G(1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity. Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex.
Cellular Location Cytoplasm. Nucleus. Membrane. Note=Cytoplasmic when non-complexed. Forms a cyclin D-CDK4 complex in the cytoplasm as cells progress through G(1) phase. The complex accumulates on the nuclear membrane and enters the nucleus on transition from G(1) to S phase. Also present in nucleoli and heterochromatin lumps. Colocalizes with RB1 after release into the nucleus
Research Areas

BACKGROUND

Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition. Phosphorylation of RB1 allows dissociation of the

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