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Anti-Vimentin Antibody

Mouse Anti Human Monoclonal Antibody

     
  • 1 - Anti-Vimentin Antibody AP53427
    Western blot detection of Vimentin in Molt-4,K562,COS7,Jurkat,Hela and Vimentin negative cell (Daudi,Ramos,Raji) lysates using Vimentin mouse mAb (1:1000 diluted).Predicted band size:57KDa.Observed band size:57KDa.
  • 8 - Anti-Vimentin Antibody AP53427
    Immunocytochemistry staining of Hela cells fixed with 4% Paraformaldehyde and using anti-Vimentin mouse mAb (dilution 1:800).
  • 5 - Anti-Vimentin Antibody AP53427
    Immunoprecipitation analysis of Hela cell lysates using Vimentin mouse mAb.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IP
Primary Accession P08670
Other Accession NM_003380
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Immunogen Purified recombinant human Vimentin protein fragments expressed in E.coli.
Purification Affinity purified
Calculated MW 57 KDa
Additional Information
Gene ID 7431
Other Names CTRCT30; Epididymis luminal protein 113; FLJ36605; HEL113; OTTHUMP00000019224; VIM; VIME_HUMAN; Vimentin.
Dilution WB~~1:1000
ICC~~1:800
Format Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name VIM
Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.
Cellular Location Cytoplasm.
Tissue Location Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone- independent mammary carcinoma cell lines
Research Areas

BACKGROUND

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

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