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Anti-β-Actin Antibody

Mouse Anti Human Monoclonal Antibody

     
  • 1 - Anti-β-Actin Antibody AP53385
    Western blot detection of beta actin in Jurkat,K562,C6,3T3 and Hela cell lysates using beta actin mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P60709
Other Accession NM_001101
Reactivity Human, Mouse, Rat
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Immunogen Purified recombinant human beta actin protein fragments expressed in E.coli.
Purification Affinity purified
Calculated MW 45 KDa
Additional Information
Gene ID 60
Other Names A X actin like protein;ACTB;ACTB_HUMAN;Actin;Actin cytoplasmic 1;Actx;b actin;b-actin;Beta cytoskeletal actin;Beta-actin;cytoplasmic 1;Melanoma X actin;N-terminally processed;PS1TP5 binding protein 1;PS1TP5BP1.
Dilution WB~~1:1000
Format Purified mouse monoclonal antibody in PB(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ACTB
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Cellular Location Cytoplasm, cytoskeleton. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs
Research Areas
Regulation of 25-hydroxyvitamin D-1-hydroxylase and 24-hydroxylase in keratinocytes by PTH and FGF23.
Author : Wu W1,Fan H2,Jiang Y3,Liao L3,Li L1,Zhao J1,Zhang H1,Shrestha C1,Xie Z1.
Exp Dermatol. 2018 Aug 1. doi: 10.1111/exd.13760. [Epub ahead of print]
30066343
Nigericin exerts anticancer effects on human colorectal cancer cells by inhibiting Wnt/β-catenin signaling pathway.
Author : Liu F1,Li W2,Hua S3,Han Y4,Xu Z4,Wan D4,Wang Y5,Chen W1,Kuang Y4,Shi J6,Zhi Q7.
Mol Cancer Ther. 2018 Feb 26. pii: molcanther.0906.2017. doi: 10.1158/1535-7163.MCT-17-0906. [Epub ahead of print]
29483216
Tumor suppressive role of miR-1224-5p in keloid proliferation, apoptosis and invasion via the TGF-β1/Smad3 signaling pathway.
Author : Yao X1,Cui X1,Wu X1,Xu P1,Zhu W1,Chen X2,Zhao T3.
Biochem Biophys Res Commun. 2017 Oct 16. pii: S0006-291X(17)32048-X. doi: 10.1016/j.bbrc.2017.10.070. [Epub ahead of print]
29050938

BACKGROUND

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

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