- 文献引用 : 3
|Application ||WB, FC, IHC-P, E|
|Other Names||CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, LAMP-3, Melanoma-associated antigen ME491, OMA81H, Ocular melanoma-associated antigen, Tetraspanin-30, Tspan-30, CD63, CD63, MLA1, TSPAN30|
|Target/Specificity||This CD63 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-190 amino acids from the C-terminal region of human CD63.|
|Format||Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.|
|Storage||Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.|
|Precautions||CD63 Antibody (C-term) is for research use only and not for use in diagnostic or therapeutic procedures.|
|Function||Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.|
|Cellular Location||Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Endosome, multivesicular body. Melanosome. Note=Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. Detected in a subset of pre-melanosomes. Detected on intralumenal vesicles (ILVs) within multivesicular bodies|
|Tissue Location||Detected in platelets (at protein level). Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages|
Author : Pan C1,Stevic I1,Müller V2,Ni Q1,Ferrer LO2,Pantel K1,Schwarzenbach H1.
Mol Oncol. 2018 Aug 14. doi: 10.1002/1878-0261.12371. [Epub ahead of print]
Author : Meng X1,Müller V2,Milde-Langosch K2,Trillsch F2,Pantel K1,Schwarzenbach H1.
Oncotarget. 2016 Mar 29;7(13):16923-35. doi: 10.18632/oncotarget.7850.
Author : García-Contreras M1, Vera-Donoso CD2, Hernández-Andreu JM1, García-Verdugo JM3, Oltra E1.
PLoS One. 2014 Nov 20;9(11):e113288. doi: 10.1371/journal.pone.0113288. eCollection 2014.
Provided below are standard protocols that you may find useful for product applications.
CD63 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene.
Weng, J., et al. J. Virol. 83(15):7467-7474(2009)
Kassahn, D., et al. Cell Death Differ. 16(1):115-124(2009)
Logozzi, M., et al. PLoS ONE 4 (4), E5219 (2009)