注册 | 登录
所有产品
  • 所有产品
  • 一抗
  • 裂解液
>   首页   >   产品   >   一抗   >   信号转导   >   PP15 Antibody   

PP15 Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - PP15 Antibody AP53312
    Anti-PP15 Antibody at 1:1000 dilution + MOLT-4 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
  • 产品详情
  • 实验流程
  • 背景知识
Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P61970
Reactivity Human, Mouse
Host Rabbit
Clonality Polyclonal
Calculated MW 14 KDa
Additional info
Other Names Nuclear transport factor 2, NTF-2, Placental protein 15, PP15, NUTF2, NTF2
Dilution WB~~ 1:1000
Format Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
StorageStore at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name NUTF2 (HGNC:13722)
Function Mediates the import of GDP-bound RAN from the cytoplasm into the nucleus which is essential for the function of RAN in cargo receptor-mediated nucleocytoplasmic transport. Thereby, plays indirectly a more general role in cargo receptor-mediated nucleocytoplasmic transport. Interacts with GDP-bound RAN in the cytosol, recruits it to the nuclear pore complex via its interaction with nucleoporins and promotes its nuclear import.
Cellular Location Cytoplasm, cytosol. Nucleus outer membrane {ECO:0000250|UniProtKB:P61972}. Nucleus, nuclear pore complex {ECO:0000250|UniProtKB:P61972}. Nucleus inner membrane {ECO:0000250|UniProtKB:P61972}. Nucleus, nucleoplasm. Note=At steady state it is essentially nucleoplasmic, enriched in nucleoplasmic foci EMBL; X07315; CAA30278.1; -; mRNA EMBL; U43939; AAA85905.1; -; mRNA EMBL; CR456937; CAG33218.1; -; mRNA EMBL; AK311822; BAG34764.1; -; mRNA EMBL; CH471092; EAW83181.1; -; Genomic_DNA EMBL; BC002348; AAH02348.1; -; mRNA CCDS; CCDS10848.1; - PIR; S00751; S00751 RefSeq; NP_001308967.1; NM_001322038.1 RefSeq; NP_001308968.1; NM_001322039.1 RefSeq; NP_001308969.1; NM_001322040.1 RefSeq; NP_001308970.1; NM_001322041.1 RefSeq; NP_005787.1; NM_005796.2 UniGene; Hs.356630; - PDB; 1GY5; X-ray; 2.30 A; A/B=1-127 PDBsum; 1GY5; - ProteinModelPortal; P61970; - SMR; P61970; - BioGrid; 115499; 53 DIP; DIP-6057N; - IntAct; P61970; 38 STRING; 9606.ENSP00000219169; - iPTMnet; P61970; - PhosphoSitePlus; P61970; - BioMuta; NUTF2; - DMDM; 48429030; - OGP; P61970; - EPD; P61970; - jPOST; P61970; - MaxQB; P61970; - PaxDb; P61970; - PeptideAtlas; P61970; - PRIDE; P61970; - ProteomicsDB; 57351; - TopDownProteomics; P61970; - DNASU; 10204; - Ensembl; ENST00000219169; ENSP00000219169; ENSG00000102898 Ensembl; ENST00000568396; ENSP00000457022; ENSG00000102898 Ensembl; ENST00000569436; ENSP00000457989; ENSG00000102898 GeneID; 10204; - KEGG; hsa:10204; - UCSC; uc002eup.4; human CTD; 10204; - DisGeNET; 10204; - EuPathDB; HostDB:ENSG00000102898.11; - GeneCards; NUTF2; - HGNC; HGNC:13722; NUTF2 HPA; HPA040915; - HPA; HPA040956; - MIM; 605813; gene neXtProt; NX_P61970; - OpenTargets; ENSG00000102898; - PharmGKB; PA38365; - eggNOG; KOG2104; Eukaryota eggNOG; ENOG4111QUX; LUCA GeneTree; ENSGT00510000047030; - HOGENOM; HOG000197024; - HOVERGEN; HBG025070; - InParanoid; P61970; - OMA; RYSQVFH; - OrthoDB; 1437819at2759; - PhylomeDB; P61970; - TreeFam; TF314422; - ChiTaRS; NUTF2; human EvolutionaryTrace; P61970; - GeneWiki; NUTF2; - GenomeRNAi; 10204; - PRO; PR:P61970; - Proteomes; UP000005640; Chromosome 16 Bgee; ENSG00000102898; Expressed in 182 organ(s), highest expression level in skeletal muscle tissue ExpressionAtlas; P61970; baseline and differential Genevisible; P61970; HS GO; GO:0005829; C:cytosol; IDA:UniProtKB GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB GO; GO:0005637; C:nuclear inner membrane; IEA:UniProtKB-SubCell GO; GO:0031965; C:nuclear membrane; ISS:UniProtKB GO; GO:0005640; C:nuclear outer membrane; IEA:UniProtKB-SubCell GO; GO:0044613; C:nuclear pore central transport channel; IBA:GO_Central GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB GO; GO:0042802; F:identical protein binding; IPI:IntAct GO; GO:0008565; F:protein transporter activity; IBA:GO_Central GO; GO:0008536; F:Ran GTPase binding; IDA:UniProtKB GO; GO:0017056; F:structural constituent of nuclear pore; IDA:GO_Central GO; GO:0051028; P:mRNA transport; IEA:UniProtKB-KW GO; GO:0006913; P:nucleocytoplasmic transport; IBA:GO_Central GO; GO:0006611; P:protein export from nucleus; IDA:BHF-UCL GO; GO:0006606; P:protein import into nucleus; IDA:UniProtKB GO; GO:0090204; P:protein localization to nuclear pore; ISS:UniProtKB CDD; cd00780; NTF2; 1 InterPro; IPR002075; NTF2 InterPro; IPR032710; NTF2-like_dom_sf InterPro; IPR018222; Nuclear_transport_factor_2_euk Pfam; PF02136; NTF2; 1 SUPFAM; SSF54427; SSF54427; 1 PROSITE; PS50177; NTF2_DOMAIN; 1 1: Evidence at protein level; 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Membrane; mRNA transport; Nuclear pore complex; Nucleus; Protein transport; Reference proteome; Translocation; Transport CHAIN 1 127 Nuclear transport factor 2 /FTId=PRO_0000194775 DOMAIN 10 121 NTF2. {ECO:0000255|PROSITE- ProRule:PRU00137} MOD_RES 4 4 N6-acetyllysine MUTAGEN 42 42 E->K: No effect on localization to the nucleoplasm MUTAGEN 124 124 Missing: No effect on localization to the nucleoplasm HELIX 6 24 {ECO:0000244|PDB:1GY5} HELIX 26 32 {ECO:0000244|PDB:1GY5} STRAND 33 41 {ECO:0000244|PDB:1GY5} STRAND 44 48 {ECO:0000244|PDB:1GY5} HELIX 49 57 {ECO:0000244|PDB:1GY5} STRAND 62 75 {ECO:0000244|PDB:1GY5} STRAND 81 94 {ECO:0000244|PDB:1GY5} STRAND 97 108 {ECO:0000244|PDB:1GY5} STRAND 111 124 {ECO:0000244|PDB:1GY5} SEQUENCE 127 AA; 14478 MW; 817752F20E262FD3 CRC64; MGDKPIWEQI GSSFIQHYYQ LFDNDRTQLG AIYIDASCLT WEGQQFQGKA AIVEKLSSLP FQKIQHSITA QDHQPTPDSC IISMVVGQLK ADEDPIMGFH QMFLLKNIND AWVCTNDMFR LALHNFG
Research Areas

BACKGROUND

Facilitates protein transport into the nucleus. Interacts with the nucleoporin p62 and with Ran. Acts at a relatively late stage of nuclear protein import, subsequent to the initial docking of nuclear import ligand at the nuclear envelope. Could be part of a multicomponent system of cytosolic factors that assemble at the pore complex during nuclear import.

REFERENCES

Grundmann U.,et al.Nucleic Acids Res. 16:4721-4721(1988).
Paschal B.M.,et al.J. Cell Biol. 129:925-937(1995).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

FeedBack

终于等到您。感谢您使用ABGENT(百奇生物)抗体产品。
点击下方“我要评价 ”按钮提交您的反馈信息,您的反馈和评价是我们最宝贵的财富之一,
我们将在1-3个工作日内处理您的反馈信息。

如有疑问,联系:0512-69552906 tech.abgent@wuxiapptec.com.


我要评价