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Eg5 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - Eg5 Antibody AP53275
    Western blot detection of Eg5 in MCF7,293T,Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa.
  • 8 - Eg5 Antibody AP53275
    Immunocytochemistry staining of Hela using Eg5 mouse mAb (1:200).
  • 5 - Eg5 Antibody AP53275
    Immunoprecipitation analysis of Jurkat cell lysates using Eg5 mouse mAb.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC, IP
Primary Accession P52732
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 130 KDa
Additional Information
Gene ID 3832
Other Names EG5;HKSP;KIF11;KIF11_HUMAN;kinesin family member 11;Kinesin like protein 1;Kinesin like spindle protein HKSP;Kinesin-like protein 1;Kinesin-like protein KIF11;Kinesin-like spindle protein HKSP;Kinesin-related motor protein Eg5;KNSL1;MCLMR;Thyroid receptor interacting protein 5;Thyroid receptor-interacting protein 5;TR-interacting protein 5; TRIP-5;TRIP5.
Dilution WB~~1:1000
ICC~~1:200
IP~~1:500
Format Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name KIF11
Synonyms EG5, KNSL1, TRIP5
Function Motor protein required for establishing a bipolar spindle during mitosis (PubMed:19001501). Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface (PubMed:23857769).
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, spindle pole
Research Areas

BACKGROUND

Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

REFERENCES

Blangy A.,et al.Cell 83:1159-1169(1995).
Whitehead C.M.,et al.J. Cell Sci. 111:2551-2561(1998).
Deloukas P.,et al.Nature 429:375-381(2004).
Lee J.W.,et al.Mol. Endocrinol. 9:243-254(1995).
Beausoleil S.A.,et al.Nat. Biotechnol. 24:1285-1292(2006).

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