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Eg5 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - Eg5 Antibody AP53275
    Western blot detection of Eg5 in MCF7,293T,Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa.
  • 8 - Eg5 Antibody AP53275
    Immunocytochemistry staining of Hela using Eg5 mouse mAb (1:200).
  • 5 - Eg5 Antibody AP53275
    Immunoprecipitation analysis of Jurkat cell lysates using Eg5 mouse mAb.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC, IP
Primary Accession P52732
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 130 KDa
Additional Information
Gene ID 3832
Other Names EG5;HKSP;KIF11;KIF11_HUMAN;kinesin family member 11;Kinesin like protein 1;Kinesin like spindle protein HKSP;Kinesin-like protein 1;Kinesin-like protein KIF11;Kinesin-like spindle protein HKSP;Kinesin-related motor protein Eg5;KNSL1;MCLMR;Thyroid receptor interacting protein 5;Thyroid receptor-interacting protein 5;TR-interacting protein 5; TRIP-5;TRIP5.
Dilution WB~~1:1000
ICC~~1:200
IP~~1:500
Format Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name KIF11
Synonyms EG5, KNSL1, TRIP5
Function Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
Cellular Location Cytoplasm. Cytoplasm, cytoskeleton, spindle pole
Research Areas

BACKGROUND

Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

REFERENCES

Blangy A.,et al.Cell 83:1159-1169(1995).
Whitehead C.M.,et al.J. Cell Sci. 111:2551-2561(1998).
Deloukas P.,et al.Nature 429:375-381(2004).
Lee J.W.,et al.Mol. Endocrinol. 9:243-254(1995).
Beausoleil S.A.,et al.Nat. Biotechnol. 24:1285-1292(2006).

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