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>   首页   >   产品   >   一抗   >   精选抗体   >   磷酸化抗体   >   Phospho--Histone H2A.X (Ser139) Monoclonal Antibody   

Phospho--Histone H2A.X (Ser139) Monoclonal Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - Phospho--Histone H2A.X (Ser139) Monoclonal Antibody AP52851
    Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.
  • 8 - Phospho--Histone H2A.X (Ser139) Monoclonal Antibody AP52851
    Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC
Primary Accession P16104
Reactivity Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 15 KDa
Additional Information
Gene ID 3014
Other Names H2A histone family, member X;H2A.X;H2a/x;H2AFX;H2AX;H2AX histone;H2AX_HUMAN;Histone H2A.X;Histone H2AX
Dilution WB~~1:2000
ICC~~1:400
Format Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name H2AFX
Synonyms H2AX
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.
Cellular Location Nucleus. Chromosome.
Research Areas

BACKGROUND

Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Required for checkpoint-mediated arrest of cell cycle progression in response to low doses of ionizing radiation and for efficient repair of DNA double strand breaks (DSBs) specifically when modified by C- terminal phosphorylation.

REFERENCES

Mannironi C.,et al.Nucleic Acids Res. 17:9113-9126(1989).
Ebert L.,et al.Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
Rogakou E.P.,et al.J. Biol. Chem. 273:5858-5868(1998).
Rogakou E.P.,et al.J. Cell Biol. 146:905-916(1999).
Paull T.T.,et al.Curr. Biol. 10:886-895(2000).

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