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STAT1 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - STAT1 Antibody AP52818
    Western blot detection of STAT1 in Hela,MCF7,COS7,SW480 and A549 cell lysates using STAT1 mouse mAb (1:1000 diluted).Predicted band size:91KDa.Observed band size:91KDa.
  • 5 - STAT1 Antibody AP52818
    Immunoprecipitation analysis of Hela cell lysates using STAT1 mouse mAb.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IP
Primary Accession P42224
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Calculated MW 91 KDa
Additional Information
Gene ID 6772
Other Names Signal transducer and activator of transcription 1 91kD;DKFZp686B04100;ISGF 3;ISGF-3;OTTHUMP00000163552;OTTHUMP00000165046; OTTHUMP00000165047;OTTHUMP00000205845;Signal transducer and activator of transcription 1 91kDa;Signal transducer and activator of transcription 1 alpha/beta;Signal transducer and activator of transcription 1;Signal transducer and activator of transcription 1, 91kD; Signal transducer and activator of transcription 1-alpha/beta;Signal Transductor and Activator of Transcription 1;STAT 1;STAT 91;Stat1; STAT1_HUMAN;STAT91;Transcription factor ISGF 3 components p91 p84;Transcription factor ISGF-3 components p91/p84.
Dilution WB~~1:1000
IP~~1:500
Format Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name STAT1
Function Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN- stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN- gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.
Cellular Location Cytoplasm. Nucleus. Note=Translocated into the nucleus upon tyrosine phosphorylation and dimerization, in response to IFN-gamma and signaling by activated FGFR1, FGFR2, FGFR3 or FGFR4
Research Areas

BACKGROUND

Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN- stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN- gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

REFERENCES

Schindler C.,et al.Proc. Natl. Acad. Sci. U.S.A. 89:7836-7839(1992).
Kristensen I.,et al.Submitted (NOV-2009) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Bechtel S.,et al.BMC Genomics 8:399-399(2007).
Kalnine N.,et al.Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.

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