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WDR77 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - WDR77 Antibody AP52808
    Western blot detection of WDR77 in C6,3T3 and K562 cell lysates using WDR77 mouse mAb (1:1000 diluted).Predicted band size:42KDa.Observed band size:42KDa.
  • 8 - WDR77 Antibody AP52808
    Immunocytochemistry staining of HeLa cells fixed in 1% Paraformaldehyde and then permeabilized in 0.1% Triton X-100,next using WDR77 mouse mAb (dilution 1:100).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC
Primary Accession Q9BQA1
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 42 KDa
Additional Information
Gene ID 79084
Other Names 2610312E17Rik;Androgen receptor cofactor p44;C79984;HKMT1069;MEP 50;MEP-50;MEP50; MEP50_HUMAN;Methylosome protein 50;MGC2722;Nbla10071;p44;p44/Mep50;RGD1310479;RP11 552M11.3;WD repeat containing protein 77;WD repeat domain 77;WD repeat-containing protein 77;WDR77.
Dilution WB~~1:1000
ICC~~1:100
Format Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name WDR77
Synonyms MEP50, WD45
Function Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.
Cellular Location Nucleus. Cytoplasm. Note=Nuclear in Leydig cells and cytoplasmic in germ cells during fetal testicular development. In adult testis, predominantly nuclear. Subcellular location varies from nuclear to cytoplasmic in various tumors
Tissue Location Highly expressed in heart, skeletal muscle, spleen, testis, uterus, prostate and thymus. In testis, expressed in germ cells and Leydig cells, but not in peritubular myocytes, nor in Sertoli cells. Expressed in prostate cancers, in seminomas and in Leydig cell tumors.
Research Areas

BACKGROUND

Non-catalytic component of the 20S PRMT5-containing methyltransferase complex, which modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins and histones. This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. Might play a role in transcription regulation. The 20S PRMT5-containing methyltransferase complex also methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage.

REFERENCES

Friesen W.J.,et al.J. Biol. Chem. 277:8243-8247(2002).
Hosohata K.,et al.Mol. Cell. Biol. 23:7019-7029(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Yamada S.,et al.Oncogene 23:5901-5911(2004).
Gregory S.G.,et al.Nature 441:315-321(2006).

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