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Smad2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - Smad2 Antibody AP52780
    Western blot detection of Smad2 in Hela,A431,Jurkat and K562 cell lysates using Smad2 mouse mAb (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.
  • 8 - Smad2 Antibody AP52780
    Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using Smad2 mouse mAb (dilution 1:100).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC
Primary Accession Q15796
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 60 KDa
Additional Information
Gene ID 4087
Other Names hMAD 2;hMAD-2;hSMAD2;JV18 1;JV18;JV18;JV18-1;JV181;MAD;MAD;MAD homolog 2;MAD Related Protein 2;Mad-related protein 2;MADH2;MADR2;MGC22139;MGC34440;Mothers Against Decapentaplegic Homolog 2;Mothers Against Decapentaplegic Homolog 2;mothers against DPP homolog 2;OTTHUMP00000163489;Sma and Mad related protein 2;SMAD 2;SMAD;SMAD family member 2;SMAD, mothers against DPP homolog 2;SMAD2;SMAD2_HUMAN.
Dilution WB~~1:500
ICC~~1:100
Format ascites
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name SMAD2
Synonyms MADH2, MADR2
Function Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4. On dephosphorylation by phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1
Tissue Location Expressed at high levels in skeletal muscle, endothelial cells, heart and placenta
Research Areas

BACKGROUND

Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD2/SMAD4 complex, activates transcription. May act as a tumor suppressor in colorectal carcinoma. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator.

REFERENCES

Riggins G.J.,et al.Nat. Genet. 13:347-349(1996).
Zhang Y.,et al.Nature 383:168-172(1996).
Eppert K.,et al.Cell 86:543-552(1996).
Liu F.,et al.Genes Dev. 11:3157-3167(1997).
Takenoshita S.,et al.Genomics 48:1-11(1998).

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