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Hsp27 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - Hsp27 Antibody AP52776
    Western blot detection of Hsp27 in Hela,HsHela,A431,COS7,Lncap and MCF7 cell lysates and using Hsp27 mouse mAb (1:1000 diluted).Predicted band size: 23KDa.Observed band size:27KDa.
  • 8 - Hsp27 Antibody AP52776
    Immunocytochemistry stain of Hela using Hsp27 mouse mAb (1:300).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC
Primary Accession P04792
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 27 KDa
Additional Information
Gene ID 3315
Other Names Heat shock 27kDa protein;28 kDa heat shock protein;CMT2F;DKFZp586P1322;Estrogen regulated 24 kDa protein;Estrogen-regulated 24 kDa protein;Heat shock 25kDa protein 1;Heat shock 27 kDa protein;Heat shock 27kD protein 1;Heat shock 27kDa protein 1;Heat shock 28kDa protein 1;Heat Shock Protein 27;Heat Shock Protein 27;Heat shock protein beta 1;Heat shock protein beta-1;HMN2B;HS.76067;Hsp 25;HSP 27;Hsp 28;Hsp B1;Hsp25;HSP27; Hsp28;HspB1;HSPB1_HUMAN;SRP27;Stress responsive protein 27;Stress-responsive protein 27.
Dilution WB~~1:1000
ICC~~1:300
Format Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide, 50% glycerol, 0.1%BSA
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name HSPB1
Synonyms HSP27, HSP28
Function Involved in stress resistance and actin organization.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Cytoplasmic in interphase cells. Colocalizes with mitotic spindles in mitotic cells. Translocates to the nucleus during heat shock and resides in sub-nuclear structures known as SC35 speckles or nuclear splicing speckles
Tissue Location Detected in all tissues tested: skeletal muscle, heart, aorta, large intestine, small intestine, stomach, esophagus, bladder, adrenal gland, thyroid, pancreas, testis, adipose tissue, kidney, liver, spleen, cerebral cortex, blood serum and cerebrospinal fluid. Highest levels are found in the heart and in tissues composed of striated and smooth muscle
Research Areas

BACKGROUND

Involved in stress resistance and actin organization.

REFERENCES

Hickey E.,et al.Nucleic Acids Res. 14:4127-4145(1986).
Carper S.W.,et al.Nucleic Acids Res. 18:6457-6457(1990).
Hino M.,et al.Biochem. Biophys. Res. Commun. 271:164-169(2000).
Briolay J.,et al.Submitted (JUN-1993) to the EMBL/GenBank/DDBJ databases.
Yu W.,et al.Genome Res. 7:353-358(1997).

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