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Pyruvate Dehydrogenase E2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - Pyruvate Dehydrogenase E2 Antibody AP52757
    Western blot detection of Pyruvate Dehydrogenase E2 in Jurkat,A549,U251,F9,Lncap and Hela cell lysates using Pyruvate Dehydrogenase E2 mouse mAb (1:1000 diluted).Predicted band size:69KDa.Observed band size:69KDa.
  • 8 - Pyruvate Dehydrogenase E2 Antibody AP52757
    Immunocytochemistry stain of Hela using Pyruvate Dehydrogenase E2 mouse mAb (1:300).
  • 5 - Pyruvate Dehydrogenase E2 Antibody AP52757
    Immunoprecipitation analysis of Hela cell lysates using Pyruvate Dehydrogenase E2 mouse mAb.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC, IP
Primary Accession P10515
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 69 KDa
Additional Information
Gene ID 1737
Other Names 70 kDa mitochondrial autoantigen of primary biliary cirrhosis;anti DLAT; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Dihydrolipoamide;Dihydrolipoamide S Acetyltransferase;Dihydrolipoamide S-acetyltransferase (E2 component of pyruvate dehydrogenase complex);Dihydrolipoamide S-Acetyltransferase;Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex;dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial;DLAT;DLAT;DLTA;E2;E2 component of pyruvate dehydrogenase complex;EC 2.3.1.12;M2 antigen complex 70 kDa subunit;M2 Antigen Complex 70kD Subunit;mitochondrial;ODP2_HUMAN;PBC;PDC E2;PDC-E2;PDCE2;Pyruvate dehydrogenase complex component E2;Pyruvate dehydrogenase complex E2 subunit;S acetyltransferase component of pyruvate dehydrogenase complex.
Dilution WB~~1:1000
ICC~~1:300
IP~~1:500
Format Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name DLAT
Synonyms DLTA
Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Cellular Location Mitochondrion matrix.
Research Areas

BACKGROUND

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.

REFERENCES

Totoki Y.,et al.Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
Taylor T.D.,et al.Nature 440:497-500(2006).
Coppel R.L.,et al.Proc. Natl. Acad. Sci. U.S.A. 85:7317-7321(1988).
Thekkumkara T.J.,et al.FEBS Lett. 240:45-48(1988).
Hiromasa Y.,et al.J. Biol. Chem. 279:6921-6933(2004).

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