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eEF2 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - eEF2 Antibody AP52728
    Western blot detection of eEF2 in HL-60,Jurkat,SHSY-5Y,U20S and Hela cell lysates using eEF2 mouse mAb (1:5000 diluted).Predicted band size:95KDa.Observed band size: 95KDa.Exposure time:15s.
  • 8 - eEF2 Antibody AP52728
    Immunocytochemistry staining of HeLa cells fixed with -20℃ Methanol and using anti-eEF2 mouse mAb (dilution 1:200).
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC
Primary Accession P13639
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Calculated MW 95 KDa
Additional Information
Gene ID 1938
Other Names EEF2;Eef2;EF-2;EF2;EF2_HUMAN;Elongation factor 2;Eukaryotic translation elongation factor 2;Polypeptidyl tRNA translocase;SCA26.
Dilution WB~~1:5000
ICC~~1:200
Format Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name EEF2
Synonyms EF2
Function Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Cellular Location Cytoplasm. Nucleus. Note=Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product.
Research Areas

BACKGROUND

Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

REFERENCES

Rapp G.,et al.Biol. Chem. Hoppe-Seyler 370:1071-1075(1989).
Hanes J.,et al.Biol. Chem. Hoppe-Seyler 373:201-204(1992).
Ustek D.,et al.Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases.
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Rapp G.,et al.Biol. Chem. Hoppe-Seyler 369:247-250(1988).

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