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B Raf Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - B Raf Antibody AP52718
    Western blot detection of B Raf in MDA-MB-468,T47D,MCF7,HCT116,C2C12,3T3 and Hela cell lysates using B Raf mouse mAb (1:1000 diluted).Predicted band size:87KDa.Observed band size:87KDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P15056
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Calculated MW 87 KDa
Additional Information
Gene ID 673
Other Names FLJ95109;94 kDa B raf protein;B raf 1;B Raf proto oncogene serine threonine protein kinase;B-Raf proto-oncogene serine/threonine-protein kinase (p94);BRAF 1;BRAF;BRAF_HUMAN;BRAF1;cRmil;MGC126806;MGC138284;Murine sarcoma viral (v-raf) oncogene homolog B1;Murine sarcoma viral v raf oncogene homolog B1;NS7;oncogene BRAF1;p94;Proto-oncogene B-Raf;Proto-oncogene c-Rmil;RAFB 1;RAFB1;RMIL;Serine/threonine-protein kinase B-raf;v raf murine sarcoma viral oncogene homolog B;v raf murine sarcoma viral oncogene homolog B1;v-Raf murine sarcoma viral oncogene homolog B1
Dilution WB~~1:1000
Format Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name BRAF
Synonyms BRAF1, RAFB1
Function Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.
Cellular Location Nucleus. Cytoplasm. Cell membrane. Note=Colocalizes with RGS14 and RAF1 in both the cytoplasm and membranes.
Tissue Location Brain and testis.
Research Areas

BACKGROUND

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

REFERENCES

Stephens R.M.,et al.Mol. Cell. Biol. 12:3733-3742(1992).
Albert S.,et al.Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
Scherer S.W.,et al.Science 300:767-772(2003).
Hillier L.W.,et al.Nature 424:157-164(2003).
Eychene A.,et al.Oncogene 7:1657-1660(1992).

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