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Rad51 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - Rad51 Antibody AP52714
    Western blot detection of Rad51 in CHO-K1,COS7,C6,3T3 and Hela cell lysates using Rad51 mouse mAb (1:1000 diluted).Predicted band size:37KDa.Observed band size:37KDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q06609
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Calculated MW 37 KDa
Additional Information
Gene ID 5888
Other Names BRCA1/BRCA2 containing complex, subunit 5 ; BRCC 5 ; BRCC5 ; DNA repair protein RAD51 homolog 1 ; DNA repair protein rhp51 ; HRAD51 ; HsRad51 ; HsT16930 ; MRMV2 ; Rad 51 ; RAD51 ; RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) ; RAD51 homolog A ; RAD51 homolog ; RAD51 recombinase ; RAD51, S. cerevisiae, homolog of ; RAD51_HUMAN ; RAD51A ; RECA ; RecA like protein ; RecA, E. coli, homolog of ; Recombination protein A.
Dilution WB~~1:1000
Format 0.03% Proclin300 and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name RAD51
Synonyms RAD51A, RECA
Function Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR) (PubMed:28575658). Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange (PubMed:26681308). Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3. Also involved in interstrand cross-link repair (PubMed:26253028).
Cellular Location Nucleus. Cytoplasm Cytoplasm, perinuclear region. Mitochondrion matrix. Chromosome. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with RAD51AP1 and RPA2 to multiple nuclear foci upon induction of DNA damage (PubMed:20154705). DNA damage induces an increase in nuclear levels (PubMed:20154705). Together with FIGNL1, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) or camptothecin (CPT) treatment (PubMed:23754376) Accumulated at sites of DNA damage in a SPIDR-dependent manner (PubMed:23509288). Recruited at sites of DNA damage in a MCM9- MCM8-dependent manner (PubMed:23401855)
Tissue Location Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary Weakly expressed in breast
Research Areas

BACKGROUND

Participates in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double-stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments. Part of a PALB2- scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. Plays a role in regulating mitochondrial DNA copy number under conditions of oxidative stress in the presence of RAD51C and XRCC3.

REFERENCES

Shinohara A.,et al.Nat. Genet. 4:239-243(1993).
Yoshimura Y.,et al.Nucleic Acids Res. 21:1665-1665(1993).
Schmutte C.,et al.Cancer Res. 59:4564-4569(1999).
Wang W.W.,et al.Cancer Epidemiol. Biomarkers Prev. 10:955-960(2001).
Park J.Y.,et al.Nucleic Acids Res. 36:3226-3234(2008).

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