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>   首页   >   产品   >   一抗   >   细胞生物学   >   DsbA-Tag Antibody   

DsbA-Tag Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - DsbA-Tag Antibody AP52698
    Western blot detection of DsbA with Dsba recombinant protein using DsbA mouse mAb (1:1000 diluted).Predicted band size: 24KDa.Observed band size: 24KDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession P0AEG4
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Calculated MW 24 KDa
Additional Information
Gene ID 12932206;948353
Other Names DSBA like thioredoxin domain containing protein.
Dilution WB~~1:1000
Format Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine(pH 7.4,150 mM NaCl)with 0.02% sodium azide,0.1mg/mlBSA and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name dsbA
Synonyms dsf, ppfA
Function Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.
Cellular Location Periplasm.
Research Areas

BACKGROUND

Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. Required for pilus biogenesis. PhoP-regulated transcription is redox-sensitive, being activated when the periplasm becomes more reducing (deletion of dsbA/dsbB, treatment with dithiothreitol). MgrB acts between DsbA/DsbB and PhoP/PhoQ in this pathway.

REFERENCES

Bardwell J.C.A.,et al.Cell 67:581-589(1991).
Kamitani S.,et al.EMBO J. 11:57-62(1992).
Plunkett G. III,et al.Nucleic Acids Res. 21:3391-3398(1993).
Blattner F.R.,et al.Science 277:1453-1462(1997).
Hayashi K.,et al.Mol. Syst. Biol. 2:E1-E5(2006).

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