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ATP-Citrate Lyase (C-terminus) Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - ATP-Citrate Lyase (C-terminus) Antibody AP52697
    Western blot detection of ATP-Citrate Lyase in 3T3,K562,COS7 and Hela cell lysates using ATP-Citrate Lyase mouse mAb (1:1000 diluted).Predicted band size: 120KDa.Observed band size: 120KDa.
  • 8 - ATP-Citrate Lyase (C-terminus) Antibody AP52697
    Immunocytochemistry of HeLa cells using anti-ATP-Citrate Lyase (C-terminus) mouse mAb diluted 1:150.
  • 4 - ATP-Citrate Lyase (C-terminus) Antibody AP52697
    Flow Cytometry analysis of HeLa cells stained with ATP-Citrate Lyase (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC, FC
Primary Accession P53396
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Calculated MW 120 KDa
Additional Information
Gene ID 47
Other Names ACL;Acly;ACLY_HUMAN;ATP citrate (pro-S) lyase;ATP citrate lyase;ATP citrate synthase; ATP-citrate (pro-S-)-lyase;ATP-citrate synthase;ATPcitrate synthase;ATPCL;Citrate cleavage enzyme;CLATP;EC 2.3.3.8;OTTHUMP00000164773.
Dilution WB~~1:1000
ICC~~1:150
FC~~1:100
Format Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name ACLY
Function ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.
Cellular Location Cytoplasm.
Research Areas

BACKGROUND

ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine.

REFERENCES

Elshourbagy N.A.,et al.Eur. J. Biochem. 204:491-499(1992).
Lord K.A.,et al.Protein Expr. Purif. 9:133-141(1997).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Potapova I.A.,et al.Biochemistry 39:1169-1179(2000).

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