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>   首页   >   产品   >   一抗   >   癌症   >   SMAD5 (C-terminus) Antibody   

SMAD5 (C-terminus) Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - SMAD5 (C-terminus) Antibody AP52696
    Western blot detection of SMAD5 (C-terminus) in Hela,Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.
  • 8 - SMAD5 (C-terminus) Antibody AP52696
    Immunocytochemistry of HeLa cells using anti-SMAD5 (C-terminus) mouse mAb diluted 1:75.
  • 4 - SMAD5 (C-terminus) Antibody AP52696
    Flow Cytometry analysis of Jurkat cells stained with SMAD5 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Black line histogram represents the isotype control, normal mouse IgG.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC, FC
Primary Accession Q99717
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 60 KDa
Additional Information
Gene ID 4090
Other Names DKFZp781C1895;DKFZp781O1323;Dwfc;hSmad 5;hSmad5;JV5 1;JV5-1;MAD homolog 5;MAD mothers against decapentaplegic homolog 5;MAD, mothers against decapentaplegic homolog 5;MADH 5;MADH5;Mothers against decapentaplegic homolog 5;Mothers against DPP homolog 5;MusMLP; SMA and MAD related protein 5;SMAD 5;SMAD family member 5;SMAD mothers against DPP homolog 5;Smad5;Smad5;SMAD5_HUMAN.
Dilution WB~~1:1000
ICC~~1:75
FC~~1:100
Format Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name SMAD5
Synonyms MADH5
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Cellular Location Cytoplasm. Nucleus. Note=Cytoplasmic in the absence of ligand. Migrates to the nucleus when complexed with SMAD4
Tissue Location Ubiquitous.
Research Areas

BACKGROUND

Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).

REFERENCES

Riggins G.J.,et al.Nat. Genet. 13:347-349(1996).
Hejlik D.P.,et al.Cancer Res. 57:3779-3783(1997).
Zavadil J.,et al.Leukemia 11:1187-1192(1997).
Gemma A.,et al.Oncogene 16:951-956(1998).
Nishimura R.,et al.J. Biol. Chem. 273:1872-1879(1998).

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