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CHD3 (C-terminus) Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - CHD3 (C-terminus) Antibody AP52685
    Western blot detection of CHD3 (C-terminus) in NTERA2,3T3,C6,K562 and Hela cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 260KDa.Observed band size: 260KDa.
  • 8 - CHD3 (C-terminus) Antibody AP52685
    Immunocytochemistry staining of HeLa cells fixed with -20℃ Methanol and using CHD3 (C-terminus) mouse mAb (dilution 1:400).
  • 5 - CHD3 (C-terminus) Antibody AP52685
    Immunoprecipitation analysis of Hela cell lysates using CHD3 mouse mAb.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC, IP
Primary Accession Q12873
Reactivity Human, Mouse
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 260 KDa
Additional Information
Gene ID 1107
Other Names 226;2600010P09Rik;AF020312;ATP dependent helicase CHD3;ATP-dependent helicase Chd3;CHD-3;Chd3;CHD3_HUMAN;Chd7;Chromodomain-helicase-DNA-binding protein 3;hZFH;MGC40857;Mi 2 autoantigen 240 kDa protein;Mi 2a;Mi-2 autoantigen 240 kDa protein;Mi2 ALPHA;Mi2-alpha; Prp7;Prp9 1;ZFH;Zinc finger helicase;zinc-finger helicase (Snf2-like).
Dilution WB~~1:1000
ICC~~1:400
IP~~1:500
Format ascites
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CHD3
Function Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
Cellular Location Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with centrosomes in interphase and mitosis
Tissue Location Widely expressed.
Research Areas

BACKGROUND

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.

REFERENCES

Aubry F.,et al.Eur. J. Biochem. 254:558-564(1998).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Woodage T.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:11472-11477(1997).
Ge Q.,et al.J. Clin. Invest. 96:1730-1737(1995).

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