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CHD3 (C-terminus) Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - CHD3 (C-terminus) Antibody AP52684
    Western blot detection of CHD3 (C-terminus) in Y79 and Raji cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 226KDa.Observed band size: 260KDa.
  • 2 - CHD3 (C-terminus) Antibody AP52684
    IHC of paraffin-embedded human colon using anti-CHD3 (C-terminus) diluted 1/500-1/1000.
  • 4 - CHD3 (C-terminus) Antibody AP52684
    Flow Cytometry analysis of K562 cells stained with CHD3 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, FC
Primary Accession Q12873
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG1
Calculated MW 260 KDa
Additional Information
Gene ID 1107
Other Names 226;2600010P09Rik;AF020312;ATP dependent helicase CHD3;ATP-dependent helicase Chd3;CHD-3;Chd3;CHD3_HUMAN;Chd7;Chromodomain-helicase-DNA-binding protein 3;hZFH;MGC40857;Mi 2 autoantigen 240 kDa protein;Mi 2a;Mi-2 autoantigen 240 kDa protein;Mi2 ALPHA;Mi2-alpha; Prp7;Prp9 1;ZFH;Zinc finger helicase;zinc-finger helicase (Snf2-like).
Dilution WB~~1:1000
IHC~~1:500
FC~~1:100
Format Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name CHD3
Function Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.
Cellular Location Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Associates with centrosomes in interphase and mitosis
Tissue Location Widely expressed.
Research Areas

BACKGROUND

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.

REFERENCES

Aubry F.,et al.Eur. J. Biochem. 254:558-564(1998).
Zody M.C.,et al.Nature 440:1045-1049(2006).
Mural R.J.,et al.Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
Woodage T.,et al.Proc. Natl. Acad. Sci. U.S.A. 94:11472-11477(1997).
Ge Q.,et al.J. Clin. Invest. 96:1730-1737(1995).

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