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TIF1 gamma / TRIM33 Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - TIF1 gamma / TRIM33 Antibody AP52681
    Western blot detection of TIF1 gamma / TRIM33 in MCF7 and HelaNE cell lysates using TIF1 gamma / TRIM33 mouse mAb (1:1000 diluted).Predicted band size: 120KDa.Observed band size: 140KDa.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB
Primary Accession Q9UPN9
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Calculated MW 140 KDa
Additional Information
Gene ID 51592
Other Names 8030451N04Rik;AI413936;cb1085;DKFZp586K1123;E3 ubiquitin-protein ligase TRIM33;EC 6.3.2.-;Ectodermin;Ectodermin homolog;FLJ11429;FLJ32925;id:ibd2175;MGC136680;mKIAA1113; OTTHUMP00000013662;OTTHUMP00000013663;Protein Rfg7;PTC7;Ret fused gene 7;RET-fused gene 7 protein;RFG7;Rfg7 protein;TF1G;TIF1-gamma;TIF1G;TIF1GAMMA;TIFGAMMA;Transcription intermediary factor 1-gamma;Transcriptional intermediary factor 1 gamma;TRI33_HUMAN; Trim33;Tripartite motif containing 33;Tripartite motif containing 33 protein;tripartite motif-containing 33;Tripartite motif-containing protein 33;wu:fc17f10;zgc:136680.
Dilution WB~~1:1000
Format Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name TRIM33
Synonyms KIAA1113, RFG7, TIF1G
Function Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade).
Cellular Location Nucleus. Note=In discrete nuclear dots resembling nuclear bodies.
Tissue Location Expressed in stem cells at the bottom of the crypts of the colon (at protein level). Expressed in colon adenomas and adenocarcinomas (at protein level). Expressed in brain, lung, liver, spleen, thymus, prostate, kidney, testis, heart, placenta, pancreas, small intestine, ovary, colon, skeletal muscle and hematopoietic progenitors
Research Areas

BACKGROUND

Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway. According to PubMed:16751102, does not promote a decrease in the level of endogenous SMAD4. May act as a transcriptional repressor. Inhibits the transcriptional response to TGF-beta/BMP signaling cascade. Plays a role in the control of cell proliferation. Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor (By similarity). Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF- beta/BMP signaling cascade).

REFERENCES

Venturini L.,et al.Oncogene 18:1209-1217(1999).
Reymond A.,et al.EMBO J. 20:2140-2151(2001).
Kikuno R.,et al.DNA Res. 6:197-205(1999).
Gregory S.G.,et al.Nature 441:315-321(2006).
Klugbauer S.,et al.Oncogene 18:4388-4393(1999).

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