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Protein Phosphatase 4C Antibody

Purified Mouse Monoclonal Antibody (Mab)

     
  • 1 - Protein Phosphatase 4C Antibody AP52663
    Western blot detection of Protein Phosphatase 4C in Hela and Jurkat cell lysates using Protein Phosphatase 4C mouse mAb (1:200 diluted).Predicted band size: 34KDa.Observed band size: 34KDa.
  • 8 - Protein Phosphatase 4C Antibody AP52663
    Immunocytochemistry of HeLa cells using anti-Protein Phosphatase 4C mouse mAb diluted 1:200.
  • 2 - Protein Phosphatase 4C Antibody AP52663
    IHC of paraffin-embedded human breast cancer using anti-Protein Phosphatase 4C mouse mAb diluted 1/500-1/1000.
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Product Information
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, ICC, IHC
Primary Accession P60510
Reactivity Human
Host Mouse
Clonality Monoclonal
Isotype IgG2a
Calculated MW 34 KDa
Additional Information
Gene ID 5531
Other Names PP X;PP-X;PP4;PP4C;PP4C;PP4C_HUMAN;PPH3;PPP4;ppp4c;PPX;protein phosphatase 4 (formerly X), catalytic subunit;Protein phosphatase 4 catalytic subunit;Protein phosphatase X; Protein phosphatase X;protein phosphatase X, catalytic subunit;Serine/threonine protein phosphatase 4 catalytic subunit;Serine/threonine-protein phosphatase 4 catalytic subunit.
Dilution WB~~1:200
ICC~~1:200
IHC~~1:100
Format Purified mouse monoclonal in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
Storage Store at -20 °C.Stable for 12 months from date of receipt
Protein Information
Name PPP4C
Synonyms PPP4, PPX
Function Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C- PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.
Cellular Location Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
Research Areas

BACKGROUND

Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C- PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.

REFERENCES

Brewis N.D.,et al.Biochim. Biophys. Acta 1171:231-233(1992).
Cohen P.T.W.,et al.Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases.
Hu M.C.-T.,et al.J. Biol. Chem. 273:33561-33565(1998).
Zhou G.,et al.J. Biol. Chem. 277:6391-6398(2002).
Mourtada-Maarabouni M.,et al.Cell Death Differ. 10:1016-1024(2003).

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