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>   首页   >   产品   >   一抗   >   癌症   >   Rabbit Anti-LEF-1 Polyclonal Antibody   

Rabbit Anti-LEF-1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Rabbit Anti-LEF-1 Polyclonal Antibody AP52338
    Mouse lymph node lysates probed with Rabbit Anti-LEF-1 Polyclonal Antibody, Unconjugated (AP52338) at 1:300 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:500 for 90 min at 37˚C.
  • 1 - Rabbit Anti-LEF-1 Polyclonal Antibody AP52338
    L1 rat brain lysates L2 human colon carcinoma lysates probed with Anti IL-2R gamma/CD132 Polyclonal Antibody, Unconjugated at 1:3000 for 90 min at 37˚C. Predicted band 44kD. Observed band size:44kD.
  • 14 - Rabbit Anti-LEF-1 Polyclonal Antibody AP52338
    Formalin-fixed and paraffin embedded rat liver tissue labeled with Anti-LEF-1 Polyclonal Antibody, Unconjugated AP52338 at 1:200 followed by conjugation to the secondary antibody, and DAB staining
  • 14 - Rabbit Anti-LEF-1 Polyclonal Antibody AP52338
    Formalin-fixed and paraffin embedded human colon carcinoma tissue labeled with Anti-LEF-1 Polyclonal Antibody, Unconjugated AP52338 at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P
Primary Accession Q9UJU2
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Additional info
Gene ID 51176
Other Names LEF-1; TCF1; TCF7L3; TCF1ALPHA; Lymphoid enhancer-binding factor 1; T cell-specific transcription factor 1-alpha; TCF1-alpha; LEF1
Dilution WB~~1/100~1/500
IHC-P~~1/100~1/500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name LEF1
Function Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1- independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.
Cellular Location Nucleus {ECO:0000255|PROSITE- ProRule:PRU00267}. Note=Found in nuclear bodies upon PIASG binding.
Tissue Location Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines
Research Areas

BACKGROUND

Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1 (By similarity). Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1- independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells.

REFERENCES

Waterman M.L.,et al.Genes Dev. 5:656-669(1991).
Hovanes K.,et al.Nucleic Acids Res. 28:1994-2003(2000).
Jesse S.,et al.Int. J. Cancer 126:1109-1120(2010).
Kobielak A.,et al.Submitted (AUG-2000) to the EMBL/GenBank/DDBJ databases.
Ota T.,et al.Nat. Genet. 36:40-45(2004).

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