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Rabbit Anti-Smac/DIABLO Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Rabbit Anti-Smac/DIABLO Polyclonal Antibody AP52328
    Lane 1: rat kidney lysates Lane 2: rat brain lysates probed with Anti AVPR2 Polyclonal Antibody, Unconjugated at 1:3000 90min in 37˚C. Predicted band 40kD. Observed band size: 40kD
  • 14 - Rabbit Anti-Smac/DIABLO Polyclonal Antibody AP52328
    Formalin-fixed and paraffin embedded mouse intestine labeled with Rabbit Anti Smac/DIABLO Polyclonal Antibody, Unconjugated (AP52328) at 1:200 followed by conjugation to the secondary antibody and DAB staining
  • 8 - Rabbit Anti-Smac/DIABLO Polyclonal Antibody AP52328
    Gastric Cancer Cells labeled with Anti-Smac/DIABLO Polyclonal Antibody, Unconjugated (AP52328) followed by conjugation to the secondary antibody
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P
Primary Accession Q9NR28
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 27131 Da
Additional info
Gene ID 56616
Other Names SMAC; DFNA64; Diablo homolog, mitochondrial; Direct IAP-binding protein with low pI; Second mitochondria-derived activator of caspase; DIABLO
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
ICC~~1:20~1:100
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name DIABLO
Synonyms SMAC
Function Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.
Cellular Location Mitochondrion. Note=Released into the cytosol when cells undergo apoptosis
Tissue Location Ubiquitously expressed with highest expression in testis. Expression is also high in heart, liver, kidney, spleen, prostate and ovary. Low in brain, lung, thymus and peripheral blood leukocytes. Isoform 3 is ubiquitously expressed
Research Areas

BACKGROUND

Promotes apoptosis by activating caspases in the cytochrome c/Apaf-1/caspase-9 pathway. Acts by opposing the inhibitory activity of inhibitor of apoptosis proteins (IAP). Inhibits the activity of BIRC6/bruce by inhibiting its binding to caspases. Isoform 3 attenuates the stability and apoptosis- inhibiting activity of XIAP/BIRC4 by promoting XIAP/BIRC4 ubiquitination and degradation through the ubiquitin-proteasome pathway. Isoform 3 also disrupts XIAP/BIRC4 interacting with processed caspase-9 and promotes caspase-3 activation. Isoform 1 is defective in the capacity to down-regulate the XIAP/BIRC4 abundance.

REFERENCES

Du C.,et al.Cell 102:33-42(2000).
Srinivasula S.M.,et al.J. Biol. Chem. 275:36152-36157(2000).
Fu J.,et al.J. Biol. Chem. 278:52660-52672(2003).
Ota T.,et al.Nat. Genet. 36:40-45(2004).
Scherer S.E.,et al.Nature 440:346-351(2006).

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