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RABBIT ANTI-PUMA POLYCLONAL ANTIBODY

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - RABBIT ANTI-PUMA POLYCLONAL ANTIBODY AP52311
    K562 lysates probed with PUMA Polyclonal Antibody, unconjugated (AP52311) at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 60 minutes at 37°C.
  • 14 - RABBIT ANTI-PUMA POLYCLONAL ANTIBODY AP52311
    Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti BBC3/PUMA Polyclonal Antibody, Unconjugated (AP52311) at 1:200 followed by conjugation to the secondary antibody and DAB staining
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P
Primary Accession Q96PG8
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 26498 Da
Additional info
Gene ID 27113
Other Names JFY1; PUMA; JFY-1; Bcl-2-binding component 3; p53 up-regulated modulator of apoptosis; BBC3
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name BBC3
Synonyms PUMA
Function Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Isoform 3 fails to show any growth- inhibitory or apoptotic activity.
Cellular Location Mitochondrion. Note=Localized to the mitochondria in order to induce cytochrome c release. Isoform 3 is not found in mitochondria
Research Areas

BACKGROUND

Essential mediator of p53/TP53-dependent and p53/TP53- independent apoptosis. Isoform 3 fails to show any growth- inhibitory or apoptotic activity.

REFERENCES

Nakano K.,et al.Mol. Cell 7:683-694(2001).
Grimwood J.,et al.Nature 428:529-535(2004).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.

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