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Rabbit Anti-CD34 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Rabbit Anti-CD34 Polyclonal Antibody AP52283
    Lane 1: human colon carcinoma Lane 2: rat kidney lysates probed with Anti CD34 Polyclonal Antibody, Unconjugated (AP52283) at 1:200 in 4˚C. Followed by conjugation to secondary antibody at 1:3000 90min in 37˚C. Predicted band 39kD. Observed band size: 39kD.
  • 14 - Rabbit Anti-CD34 Polyclonal Antibody AP52283
    Formalin-fixed and paraffin embedded human gastric carcinoma tissue labeled with Anti-CD34 Polyclonal Antibody, Unconjugated(AP52283) at 1:200 followed by conjugation to the secondary antibody and DAB staining
  • 3 - Rabbit Anti-CD34 Polyclonal Antibody AP52283
    Formalin-fixed and paraffin embedded human gastric adenocarcinoma tissue labeled with Anti-CD34 Polyclonal Antibody, Unconjugated AP52283 at 1:200 followed by conjugation to the secondary antibody, Goat Anti-Rabbit IgG, Cy3 conjugated, and DAPI staining
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, ICC, IHC-P
Primary Accession Q64314
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Additional info
Gene ID 12490
Other Names AU496; Hematopoietic progenitor cell antigen CD34; CD34
Dilution WB~~1/100~1/500
IHC-P~~1/100~1/500
IF~~1/100~1/200
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name Cd34
Function Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Highly expressed in hematopoietic progenitor cell lines, brain and testis, and moderately in the thymus, spleen, and bone marrow, but not in adult liver
Research Areas

BACKGROUND

Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins (By similarity).

REFERENCES

Brown J.,et al.Int. Immunol. 3:175-184(1991).
Suda J.,et al.Blood 79:2288-2295(1992).
Rush J.,et al.Nat. Biotechnol. 23:94-101(2005).
Cao L.,et al.J. Immunol. 179:5864-5876(2007).
Gundry R.L.,et al.Mol. Cell. Proteomics 8:2555-2569(2009).

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