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>   首页   >   产品   >   一抗   >   心血管   >   Rabbit Anti-CX3CL1 Polyclonal Antibody   

Rabbit Anti-CX3CL1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Rabbit Anti-CX3CL1 Polyclonal Antibody AP52235
    L1 rat heart lysates L2 rat brain lysates probed with Anti CX3CL1 Polyclonal Antibody, Unconjugated (AP52235) at 1:200 overnight at 4˚C. Followed by conjugation to secondary antibody at 1:3000 for 90 min at 37˚C. Predicted band 41kD. Observed band size:41/70kD.
  • 14 - Rabbit Anti-CX3CL1 Polyclonal Antibody AP52235
    Paraformaldehyde-fixed, paraffin embedded human kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-CX3CL1 Polyclonal Antibody, Unconjugated (AP52235) at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining.
  • 4 - Rabbit Anti-CX3CL1 Polyclonal Antibody AP52235
    Human A549 cell lysates probed with Rabbit Anti-CX3CL1 Polyclonal Antibody, Unconjugated (AP52235) (green) at 1:10 for 30 minutes followed by a FITC conjugated secondary antibody compared to unstained cells (blue).
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P
Primary Accession P78423
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 42203 Da
Additional info
Gene ID 6376
Other Names NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin; C-X3-C motif chemokine 1; CX3C membrane-anchored chemokine; Small-inducible cytokine D1; CX3CL1; FKN; A-152E5.2
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
FC~~0.0486111111111
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name CX3CL1
Synonyms FKN, NTT, SCYD1
Function The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.
Cellular Location Cell membrane; Single-pass type I membrane protein
Tissue Location Small intestine, colon, testis, prostate, heart, brain, lung, skeletal muscle, kidney and pancreas
Research Areas

BACKGROUND

The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.

REFERENCES

Bazan J.F.,et al.Nature 385:640-644(1997).
Loftus B.J.,et al.Genomics 60:295-308(1999).
Nilsson J.,et al.Nat. Methods 6:809-811(2009).
Halim A.,et al.Mol. Cell. Proteomics 0:0-0(2011).
Mizoue L.S.,et al.Biochemistry 38:1402-1414(1999).

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