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Rabbit Anti-CD68 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - Rabbit Anti-CD68 Polyclonal Antibody AP52189
    rat myocardium tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-CD68 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining
  • 1 - Rabbit Anti-CD68 Polyclonal Antibody AP52189
    Western blot analysis of extracts from Bone tissue(mouse) using CD68 Antibody.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IHC, IHC-P
Primary Accession P31996
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Additional info
Gene ID 12514
Other Names Lamp4; gp11; Scard1; Macrosialin; CD68
Dilution IHC-P~~1/100~1/500
WB~~1:100-1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name Cd68
Function Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.
Cellular Location Isoform Long: Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein
Tissue Location Expressed in tissue macrophages and to a lesser extent in dendritic cells
Research Areas
Glaucocalyxin A improves survival in bleomycin-induced pulmonary fibrosis in mice.
Author : Yang F1,Cao Y1,Zhang J2,You T1,Zhu L3.
Biochem Biophys Res Commun. 2016 Nov 3. pii: S0006-291X(16)31851-4. doi: 10.1016/j.bbrc.2016.11.003. [Epub ahead of print]
27816453

BACKGROUND

Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells.

REFERENCES

Holness C.L.,et al.J. Biol. Chem. 268:9661-9666(1993).
Jiang Z.,et al.Genomics 50:199-205(1998).
Greaves D.R.,et al.Genomics 54:165-168(1998).
Li A.C.,et al.J. Biol. Chem. 273:5389-5399(1998).
Miyashita A.,et al.Gene 237:53-60(1999).

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