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>   首页   >   产品   >   一抗   >   癌症   >   Rabbit Anti-N-cadherin Polyclonal Antibody   

Rabbit Anti-N-cadherin Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Rabbit Anti-N-cadherin Polyclonal Antibody AP52149
    Mouse breast cancer lysates probed with Anti-N-cadherin Polyclonal Antibody, Unconjugated (AP52149) at 1:300 in 4˚C. Followed by conjugation to secondary antibody at 1:5000 90min in 37˚C.
  • 14 - Rabbit Anti-N-cadherin Polyclonal Antibody AP52149
    Paraformaldehyde-fixed, paraffin embedded human lung carcinoma tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-N-cadherin Polyclonal Antibody, Unconjugated (AP52149) at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining
  • 14 - Rabbit Anti-N-cadherin Polyclonal Antibody AP52149
    Formalin-fixed and paraffin embedded rat brain labeled with Anti-CDH2/N-cadherin Polyclonal Antibody, Unconjugated (AP52149) at 1:200 followed by conjugation to the secondary antibody and DAB staining.
  • 3 - Rabbit Anti-N-cadherin Polyclonal Antibody AP52149
    Formalin-fixed and paraffin-embedded rat brain labeled with Anti-CDH2/N-cadherin Polyclonal Antibody, Unconjugated(AP52149) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, PE conjugated secondary antibody used at 1:200 dilution for 40 minutes at 37°C.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P
Primary Accession P19022
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Additional info
Gene ID 1000
Other Names CDHN; NCAD; CD325; CDw325; Cadherin-2; Neural cadherin; N-cadherin; CDH2
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
IF~~1:100~1:200
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name CDH2
Synonyms CDHN, NCAD
Function Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Acts as a regulator of neural stem cells quiescence by mediating anchorage of neural stem cells to ependymocytes in the adult subependymal zone: upon cleavage by MMP24, CDH2-mediated anchorage is affected, leading to modulate neural stem cell quiescence. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).
Cellular Location Cell membrane; Single-pass type I membrane protein
Research Areas
MiR-5100 targets TOB2 to drive epithelial-mesenchymal transition associated with activating smad2/3 in lung epithelial cells.
Author : Li CY1,Wang YH2,Lin ZY2,Yang LW2,Gao SL2,Liu T3,Zou BA1,Pan ZC1,Song ZQ1,Liu G2,1.
Am J Transl Res. 2017 Oct 15;9(10):4694-4706. eCollection 2017.
29118928

BACKGROUND

Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density (By similarity).

REFERENCES

Reid R.A.,et al.Nucleic Acids Res. 18:5896-5896(1990).
Reid R.A.,et al.Submitted (NOV-1990) to the EMBL/GenBank/DDBJ databases.
Salomon D.,et al.J. Cell Sci. 102:7-17(1992).
Mural R.J.,et al.Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
Walsh F.S.,et al.J. Neurochem. 55:805-812(1990).

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