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Rabbit Anti-ICAM1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 1 - Rabbit Anti-ICAM1 Polyclonal Antibody AP52125
    Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti CD54/ICAM-1 Polyclonal Antibody, Unconjugated (AP52125) at 1:200 in 4˚C. Followed by conjugation to secondary antibody at 1:3000 90min in 37˚C. Predicted band 60kD. Observed band size: 60kD.
  • 14 - Rabbit Anti-ICAM1 Polyclonal Antibody AP52125
    Formalin-fixed and paraffin embedded rat brain tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody (AP52125), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
  • 3 - Rabbit Anti-ICAM1 Polyclonal Antibody AP52125
    Formalin-fixed and paraffin embedded mouse heart tissue labeled with Anti-CD54/ICAM-1 Polyclonal Antibody (AP52125), Unconjugated at 1:200, followed by conjugation to the secondary antibody Goat Anti-Rabbit IgG, FITC conjugated(bs-0295G-FITC)used at 1:200 dilution for 40 minutes at 37°C and DAPI staining
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P
Primary Accession P13597
Reactivity Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 58844 Da
Additional info
Gene ID 15894
Other Names CD54; Ly-47; Icam-1; MALA-2; Intercellular adhesion molecule 1; MyD1; Icam1
Dilution WB~~1:100~1:500
IHC-P~~1:100~1:500
IF~~1:100~1:200
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name Icam1
Synonyms Icam-1
Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
Cellular Location Membrane; Single-pass type I membrane protein
Tissue Location Expressed at low level on a subpopulation of lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells, and on fibroblasts and epithelial cells
Research Areas

BACKGROUND

ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans- endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).

REFERENCES

Horley K.J.,et al.EMBO J. 8:2889-2896(1989).
Takei F.,et al.Submitted (APR-1990) to the EMBL/GenBank/DDBJ databases.
Siu G.,et al.J. Immunol. 143:3813-3820(1989).
Ballantyne C.M.,et al.Nucleic Acids Res. 17:5853-5853(1989).
Ballantyne C.M.,et al.Genomics 14:1076-1080(1992).

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