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Rabbit Anti-MyoD1 Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - Rabbit Anti-MyoD1 Polyclonal Antibody AP52107
    Formalin-fixed and paraffin embedded rat embryonic rhabdomyoma labeled with Anti-MyoD1/Myf3 Polyclonal Antibody, Unconjugated (AP52107) at 1:200 followed by conjugation to the secondary antibody
  • 1 - Rabbit Anti-MyoD1 Polyclonal Antibody AP52107
    Lane 1: mouse muscle lysates; Lane 2: mouse heart lysates probed with MyoD1 Polyclonal Antibody, Unconjugated (AP52107) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, ICC, IHC-P
Primary Accession P10085
Reactivity Human, Mouse, Rat
Host Rabbit
Clonality Polyclonal
Calculated MW 34233 Da
Additional info
Gene ID 17927
Other Names MYF3; MyoD; Myod-1; bHLHc1; AI53393; Myoblast determination protein 1; Myod1
Dilution IHC-P~~1:100~1:500
WB~~1:100~1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name Myod1
Synonyms Myod
Function Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.
Cellular Location Nucleus.
Research Areas

BACKGROUND

Acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. Together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. Interacts with and is inhibited by the twist protein. This interaction probably involves the basic domains of both proteins.

REFERENCES

Davis R.L.,et al.Cell 51:987-1000(1987).
Zingg J.-M.,et al.Nucleic Acids Res. 19:6433-6439(1991).
Pinney D.F.,et al.Cell 53:781-793(1988).
Carninci P.,et al.Science 309:1559-1563(2005).
Tapscott S.J.,et al.Science 242:405-411(1988).

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