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>   首页   >   产品   >   一抗   >   细胞生物学   >   Rabbit Anti-Ubiquitin Polyclonal Antibody   

Rabbit Anti-Ubiquitin Polyclonal Antibody

Purified Rabbit Polyclonal Antibody (Pab)

     
  • 14 - Rabbit Anti-Ubiquitin Polyclonal Antibody AP52106
    Formalin-fixed and paraffin embedded human breast cancer labeled with Anti-Ubiquitin/UBC/UB Polyclonal Antibody, Unconjugated (AP52106) followed by conjugation to the secondary antibody and DAB staining
  • 1 - Rabbit Anti-Ubiquitin Polyclonal Antibody AP52106
    Ubiquitin protein probed with Rabbit Anti-Ubiquitin Polyclonal Antibody, Unconjugated (AP52106) at 1:300 overnight at 4˚C. Followed by a conjugated secondary antibody at 1:5000 for 90 min at 37˚C.
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Product info
Application
  • Applications Legend:
  • E=ELISA
  • WB=Western Blotting
  • IHC=Immunohistochemistry
  • IHC-P=Immunohistochemistry (Paraffin)
  • IP=Immunoprecipitation
  • IF=Immunofluorescence
  • IC=Immunochemistry
  • ICC=Immunocytochemistry
  • FC=Flow Cytometry
  • DB=Dot Blot
WB, IF, IHC-P
Primary Accession P0CG48
Reactivity Human, Mouse, Rat, Pig
Host Rabbit
Clonality Polyclonal
Calculated MW 77039 Da
Additional info
Gene ID 7316
Other Names HMG2; Polyubiquitin-C; UBC
Dilution IHC-P~~1:100~1:500
WB~~1:100~1:500
Format0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
StorageStore at -20 °C for one year. Avoid repeated freeze/thaw cycles. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Protein Information
Name UBC
Function Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
Cellular Location Ubiquitin: Cytoplasm. Nucleus
Research Areas

BACKGROUND

Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.

REFERENCES

Wiborg O.,et al.EMBO J. 4:755-759(1985).
Kim N.S.,et al.J. Biochem. 124:35-39(1998).
Tachikui H.,et al.J. Mol. Evol. 57:737-744(2003).
Scherer S.E.,et al.Nature 440:346-351(2006).
Nenoi M.,et al.Gene 175:179-185(1996).

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